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Title:
HIGHLY EFFICIENT AND CONTROLLABLE EXPRESSION SYSTEM FOR ENDOGENOUS PIGGYBACKING EXOGENEOUS GENES
Document Type and Number:
WIPO Patent Application WO/2021/088370
Kind Code:
A1
Abstract:
Provided is a fungal operon, an exogeneous gene site-directed quantitative fixed-time expression system based on the fungal operon, and applications of the system in expressing heterogeneous genes. The system comprises the following sequences connected in series: a host target gene stop codon upstream fragment containing a stop codon, a fungal operon intergenic region, a target gene, and a DNA fragment of a host target gene stop codon downstream fragment. The system utilizes the fungal operon intergenic region to serially connect the target gene, inserts same to the downstream of a host cell expression gene stop codon by means of site-directed gene insertion, and utilizes a promoter of the target gene to complete the transcriptional expression of the target gene.

Inventors:
XU YUQUAN (CN)
YUE QUN (CN)
ZHANG LIWEN (CN)
Application Number:
PCT/CN2020/096010
Publication Date:
May 14, 2021
Filing Date:
June 14, 2020
Export Citation:
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Assignee:
BIOTECHNOLOGY RES INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CN)
International Classes:
C12N15/113; C12N1/19; C12N15/81; C12R1/865
Domestic Patent References:
WO2004046361A12004-06-03
Foreign References:
CN111088254A2020-05-01
CN104540946A2015-04-22
CN107090467A2017-08-25
Other References:
YUE QUN, CHEN LI, LI YAN, BILLS GERALD F., ZHANG XINYU, XIANG MEICHUN, LI SHAOJIE, CHE YONGSHENG, WANG CHENGSHU, NIU XUEMEI, AN ZH: "Functional Operons in Secondary Metabolic Gene Clusters in Glarea lozoyensis(Fungi, Ascomycota, Leotiomycetes)", MBIO, vol. 6, no. 3, 16 June 2015 (2015-06-16), pages 1 - 10, XP055810344, ISSN: 2150-7511, DOI: 10.1128/mBio.00703-15
Attorney, Agent or Firm:
BEIJING ZHIBENCUN INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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