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Title:
IMMOBILISED ENZYME METHOD FOR IMPROVING THE STABILITY OF HORSERADISH PEROXIDASE, AND APPLICATION THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/228291
Kind Code:
A1
Abstract:
Provided are an immobilised enzyme method for improving the stability of horseradish peroxidase, and an application therefor. At a normal temperature and pressure, a co-precipitation method is used to synthesise a biological composite of zeolite imidazolate framework-8/graphene immobilised natural horseradish peroxidase in the aqueous phase. Embedding of ZIF-8/GO composite material retains the peroxidase catalytic activity of HRP and enhances the stability of HRP. Relative to a separate ZIF-8 mineralised HRP material, the introduction of GO increases the resistance of HRP to complex environments such as high temperatures and organic solvents and denaturants, and also enhances the storage stability and recycling capability of HRP. DNA molecules are used to regulate the peroxidase catalytic activity of the HRP@ZIF-8/GO biological composite material, and a label-free colorimetric sensing method is established to implement the specific detection of kanamycin.

Inventors:
ZHAO HUIMIN (CN)
TAN BING (CN)
Application Number:
PCT/CN2019/119108
Publication Date:
November 19, 2020
Filing Date:
November 18, 2019
Export Citation:
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Assignee:
UNIV DALIAN TECH (CN)
International Classes:
C12N9/04; C12N11/14; C12N9/96; C12N11/02; G01N21/31; G01N21/78
Foreign References:
CN110218720A2019-09-10
CN103290124A2013-09-11
CN105734038A2016-07-06
CN105734038A2016-07-06
Other References:
FAN, Z.J. ET AL.: "Metal-Organic Frameworks/Graphene Oxide Composite: A New Enzymatic Immobilization Carrier for Hydrogen Peroxide Biosensors. Journal of The Electrochemical Society", JOURNAL OF THE ELECTROCHEMICAL SOCIETY, vol. 163, no. 3, 19 December 2015 (2015-12-19), XP055753311, DOI: 20200215111956X
FAN, Z.J. ET AL.: "Metal-Organic Frameworks/Graphene Oxide Composite: A New Enzymatic Immobilization Carrier for Hydrogen Peroxide Biosensors. Journal of The Electrochemical Society", JOURNAL OF THE ELECTROCHEMICAL SOCIETY, vol. 163, no. 3, 19 December 2015 (2015-12-19), XP055753311, DOI: 20200215105921Y
LIU, J.W. ET AL.: "Tunable Composites Prepared from Graphene Oxide and Zeolitic Imidazolate Framework-8 for Improved Selective Isolation of Hemoglobin.", MICROCHIMICA ACTA., vol. 185, 5 July 2018 (2018-07-05), XP036570198, DOI: 20200215110537X
LIU, JIAWEI ET AL.: "Tunable Composites Prepared from Graphene Oxide and Zeolitic Imidazolate Framework-8 for Improved Selective Isolation of Hemoglobin.", MICROCHIMICA ACTA., vol. 185, 5 July 2018 (2018-07-05), XP036570198, DOI: 20200215110544Y
KANOKPHANDHARANGKUL, K.: "Aptamer Biosensors.", THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF IMPERIAL COLLEGE LONDON., 30 April 2015 (2015-04-30), DOI: 20200215111250Y
LIU, XINJIA ET AL.: "Development of Enzyme-linked Aptamer Assay for Detection of Kanamycin A", CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, vol. 41, no. 9, 30 September 2013 (2013-09-30), DOI: 20200215111452Y
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