Title:
IMMUNOCHROMATOGRAPHY
Document Type and Number:
WIPO Patent Application WO/2021/065144
Kind Code:
A1
Abstract:
Provided is immunochromatography having a high detection sensitivity. The immunochromatography involves: a mixing step of mixing a sample that may contain an antigen with modified magnetic particles which are magnetic particles modified with a substance having an affinity specifically for the antigen, thereby obtaining a mixture containing a magnetic particle complex; a collection step of using magnetism to collect the magnetic particles; a separation step of mixing the collected magnetic particles with a separation solution in an amount that is less than the sample that may contain the antigen to separate the modified magnetic particles and obtain an antigen concentrate solution; a neutralization step of obtaining a neutralized antigen concentrate solution; a development step of forming a gold particle complex, which is a complex between the antigen in the neutralized antigen concentrate solution and modified gold particles that are gold particles modified with a first binding substance, and in this state, proceeding with development into an insoluble support having a reactive site wherein a second binding substance has been fixed; a trapping step of trapping the gold particle complex in the reactive site; and a silver enhancement step of enhancing the gold particle complex with silver.
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Inventors:
ABURAYA YOSHIHIRO (JP)
NISHIKAWA NAOYUKI (JP)
KATADA JUNICHI (JP)
WADA ATSUHIKO (JP)
UJIHARA DAI (JP)
NISHIKAWA NAOYUKI (JP)
KATADA JUNICHI (JP)
WADA ATSUHIKO (JP)
UJIHARA DAI (JP)
Application Number:
PCT/JP2020/027056
Publication Date:
April 08, 2021
Filing Date:
July 10, 2020
Export Citation:
Assignee:
FUJIFILM CORP (JP)
International Classes:
G01N33/543; G01N33/53
Domestic Patent References:
| WO2019117518A1 | 2019-06-20 | |||
| WO2013129634A1 | 2013-09-06 | |||
| WO2017139153A1 | 2017-08-17 | |||
| WO2013129634A1 | 2013-09-06 |
Foreign References:
| CN105116146A | 2015-12-02 | |||
| JPH02118451A | 1990-05-02 | |||
| JP2013213803A | 2013-10-17 | |||
| JP2016090570A | 2016-05-23 | |||
| JPH0843391A | 1996-02-16 | |||
| JPS5728453B2 | 1982-06-16 | |||
| US6020117A | 2000-02-01 | |||
| JP2009150869A | 2009-07-09 |
Other References:
FISHER, M., ATIYA-NASAGI Y., SIMON I., GORDIN M., MECHALY A., YITZHAKI S.: "A combined immunomagnetic separation and lateral flow method for a sensitive on-site detection of Bacillus anthracis spores-assessment in water and dairy products", LETTERS IN APPLIED MICROBIOLOGY, vol. 48, no. 4, 9 March 2009 (2009-03-09), pages 413 - 418, XP055813925, DOI: 10.1111/j.1472-765X.2008.02542.x
LAWN, S.D.: "Point-of-care detection of lipoarabinomannan (LAM) in urine for diagnosis of HIV-associated tuberculosis : a state of the art review", BMC INFECT DIS., vol. 12, 26 April 2012 (2012-04-26), pages 103, XP021132590, DOI: 10.1186/1471-2334-12-103
See also references of EP 4040155A4
LAWN, S.D.: "Point-of-care detection of lipoarabinomannan (LAM) in urine for diagnosis of HIV-associated tuberculosis : a state of the art review", BMC INFECT DIS., vol. 12, 26 April 2012 (2012-04-26), pages 103, XP021132590, DOI: 10.1186/1471-2334-12-103
See also references of EP 4040155A4
Attorney, Agent or Firm:
NAKASHIMA Junko et al. (JP)
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