Title:
IMMUNOSUPPRESSIVE FACTOR
Document Type and Number:
WIPO Patent Application WO/1987/006591
Kind Code:
A1
Abstract:
Immunosuppressive factor of the following properties: (1) molecular weight: 45,000 to 65,000 and 150,000 to 200,000 according to gel filtration and 31,000 according to SDS-polyacrylamide gel electrophoresis; (2) isoelectric point: 4.6 to 4.8; (3) being elutable at a salt concentration of 0.31 to 0.32 M by FPLC-mono Q anion exchange chromatography; (4) not adsorbable to immobilized concanavalin A sepharose and Blue sepharose; (5) non-sensitive to deoxyribonuclease, ribonuclease, papain, and periodic acid, and sensitive to trypsin, alpha-chymotrypsin, and pronase; (6) stable at pH 2 to 10; (7) stable for a long time at 4�C and partly deactivated by heat treatment at 56�C or 90�C for 30 minutes; (8) not inhibitable by 2-mercaptoethanol, levamisole, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, alpha-methyl-D-mannose, L-arginine or L-ornithine; (9) inhibiting production of antibody; (10) inhibiting blastogenic transformation of lymphocytes; (11) inhibiting cell division and cell multiplication; and (12) having specific structural amino acids and specific contents thereof. This immunosuppressive factor has an excellent immunosuppressing effect and is useful as medicine or reagent.
Inventors:
HASHIMOTO YOSHIYUKI (JP)
CHIBA KENJI (JP)
KOMATSU HIROTSUGU (JP)
OKUMOTO TAKEKI (JP)
CHIBA KENJI (JP)
KOMATSU HIROTSUGU (JP)
OKUMOTO TAKEKI (JP)
Application Number:
PCT/JP1987/000268
Publication Date:
November 05, 1987
Filing Date:
April 27, 1987
Export Citation:
Assignee:
YOSHITOMI PHARMACEUTICAL (JP)
International Classes:
C12P21/00; A61K35/12; A61K38/00; A61P37/06; C07K14/005; C07K14/195; C07K14/52; C12R1/91; (IPC1-7): C07K15/04; C07K15/06
Domestic Patent References:
| WO1985004421A1 | 1985-10-10 |
Foreign References:
| JPH05888320A | ||||
| JPS58138395A | 1983-08-17 | |||
| JPS59116224A | 1984-07-05 |
Other References:
See also references of EP 0269742A4
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