ARMIGER WILLIAM B (US)
DODDS DAVID R (US)
KOFFAS MATTHEOS (US)
WO2014039767A1 | 2014-03-13 | |||
WO2016070168A1 | 2016-05-06 |
US20150228996A1 | 2015-08-13 | |||
CA1207691A | 1986-07-15 | |||
US6270649B1 | 2001-08-07 | |||
US7250288B2 | 2007-07-31 | |||
US4705704A | 1987-11-10 | |||
US5077217A | 1991-12-31 |
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See also references of EP 3430155A4
CLAIMS 1. A system for electrochemically generating NAD(P)H2 reducing equivalents comprising: a. an electrochemical cell comprising an anode contained in an anode chamber and a cathode contained in a cathode chamber; b. a first process stream containing NAD(P), passing through the cathode chamber and continuously in contact with the cathode from which electrons are transferred to the NAD(P) to produce a second process stream containing reduced species 1,4‐NAD(P)H2, 1,2‐NAD(P)H2, 1,6‐ NAD(P)H2, and [NAD(P)]2, while optionally producing hydrogen; c. a substrate of an oxidoreductase or P450 enzyme, which, when contacted with the second process stream in the presence of the oxidoreductase or P450 enzyme, is transformed to a product while concomitantly consuming the 1,4‐NAD(P)H2 in the second process stream and producing a first recovered NAD(P) and a third process stream; and d. at least one of a renalase enzyme, a Mung Bean Phenol Oxidase enzyme and illumination at a wavelength of about 254 nm or exceeding about 320 nm, which, when contacted with the third process stream, convert at least one of the 1,2‐NAD(P)H2, 1,6‐NAD(P)H2, and [NAD(P)]2 therein to a second and optionally a third recovered NAD(P). 2. The system of claim 1, comprising the renalase enzyme for converting the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 into the second recovered NAD(P). 3. The system of claim 1, comprising the Mung Bean Phenol Oxidase enzyme for converting the 1,2‐NAD(P)H2, 1,6‐NAD(P)H2, and/or [NAD(P)]2 into the second recovered NAD(P) and/or the third recovered NAD(P). 4. The system of claim 1, comprising the illumination for converting the [NAD(P)]2 into NAD(P). 5. The system of claim 1, comprising the renalase enzyme and the Mung Bean Phenol Oxidase enzyme. 6. The system of claim 5, wherein the third process stream is contacted with the renalase enzyme resulting in conversion of the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 into the second recovered NAD(P) and a fourth process stream, wherein the fourth process stream is contacted with the Mung Bean Phenol Oxidase enzyme to convert the [NAD(P)]2 therein to the third recovered NAD(P). 7 The system of claim 1 comprising the renalase enzyme and the illumination 8. The system of claim 7, wherein the third process stream is contacted with the renalase enzyme resulting in conversion of the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 into the second recovered NAD(P) and a fourth process stream, wherein the fourth process stream is contacted with the illumination to convert the [NAD(P)]2 therein to the third recovered NAD(P). 9. The system of claim 1, comprising the Mung Bean Phenol Oxidase enzyme and the illumination. 10. The system of claim 8, wherein the third process stream is contacted with the Mung Bean Phenol Oxidase enzyme resulting in conversion of the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 into the second recovered NAD(P) and a fourth process stream, wherein the fourth process stream is contacted with the illumination to convert the [NAD(P)]2 therein to the third recovered NAD(P). 11. The system of any one of claims 1‐10, further comprising, in one or more of the process streams, an electron transfer mediator (ETM) capable of transferring electrons to NAD(P). 12. The system of any one of claims 1‐10, further comprising catalase for decomposing hydrogen peroxide produced by the renalase enzyme, the Mung Bean Phenol Oxidase enzyme, and/or the illumination. 13. The system of any one of claims 1‐10, wherein the renalase enzyme is a mutant form having a lower oxidation activity for 1,4‐NAD(P)H2 than the wild type renalase enzyme, and/or a higher oxidation activity for 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 than the wild type renalase enzyme, wherein preferably the mutant form is recombinantly expressed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium. 14. The system of any one of claims 1‐10, wherein the renalase enzyme and/or Mung Bean Phenol Oxidase enzyme is recombinantly expressed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium. 15. The system of any one of claims 1‐10, wherein the Mung Bean Phenol Oxidase enzyme is a mutant form having a lower oxidation activity for 1,4‐NAD(P)H2 than the wild type Mung Bean Phenol Oxidase enzyme, and/or a higher oxidation activity for 1,2‐NAD(P)H2, 1,6‐NAD(P)H2 and/or [NAD(P)]2 than the wild type Mung Bean Phenol Oxidase enzyme, wherein preferably the mutant form is recombinantly expressed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus 16. A method for electrochemically generating NAD(P)H2 reducing equivalents comprising: a. providing an electrochemical cell comprising an anode contained in an anode chamber and a cathode contained in a cathode chamber; b. passing through the cathode chamber a first process stream which contains NAD(P) and is continuously in contact with the cathode from which electrons are transferred to the NAD(P) to produce a second process stream containing reduced species 1,4‐NAD(P)H2, 1,2‐ NAD(P)H2, 1,6‐NAD(P)H2, and [NAD(P)]2, while optionally producing hydrogen; c. contacting the second process stream with a substrate of an oxidoreductase or P450 enzyme such that the substrate, in the presence of the oxidoreductase or P450 enzyme, is transformed to a product while concomitantly consuming the 1,4‐NAD(P)H2 in the second process stream and producing a first recovered NAD(P) and a third process stream; and d. contacting the third process stream with at least one of a renalase enzyme, a Mung Bean Phenol Oxidase enzyme and illumination at a wavelength of about 254 nm or exceeding about 320 nm, thereby converting at least one of the 1,2‐NAD(P)H2, 1,6‐NAD(P)H2, and [NAD(P)]2 therein to a second recovered NAD(P) and optionally a third recovered NAD(P). 17. The method of claim 16, further comprising contacting the third process stream with the renalase enzyme for converting the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 into the second recovered NAD(P). 18. The method of claim 16, further comprising contacting the third process stream with the Mung Bean Phenol Oxidase enzyme for converting the 1,2‐NAD(P)H2, 1,6‐NAD(P)H2, and/or [NAD(P)]2 into the second recovered NAD(P) and/or the third recovered NAD(P). 19. The method of claim 16, further comprising contacting the third process stream with the illumination for converting the [NAD(P)]2 into NAD(P). 20. The method of claim 16, further comprising contacting the third process stream with the renalase enzyme resulting in conversion of the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 therein into the second recovered NAD(P) and a fourth process stream, and further comprising contacting the fourth process stream with the Mung Bean Phenol Oxidase enzyme to convert the [NAD(P)]2 therein to the third recovered NAD(P). 21. The method of claim 16, further comprising contacting the third process stream with the renalase enzyme resulting in conversion of the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 therein into the second recovered NAD(P) and a fourth process stream, and further comprising contacting the recovered NAD(P). 22. The method of claim 16, further comprising contacting the third process stream with the Mung Bean Phenol Oxidase enzyme resulting in conversion of the 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 therein into the second recovered NAD(P) and a fourth process stream, and further comprising contacting the fourth process stream with the illumination to convert the [NAD(P)]2 therein to the third recovered NAD(P). 23. The method of any one of claims 16‐22, further comprising providing, in one or more of the process streams, an electron transfer mediator (ETM) capable of transferring electrons to NAD(P). 24. The method of any one of claims 16‐22, further comprising providing catalase for decomposing hydrogen peroxide produced by the renalase enzyme, the Mung Bean Phenol Oxidase enzyme, and/or the illumination. 25. The method of any one of claims 16‐22, wherein the renalase enzyme is a mutant form having a lower oxidation activity for 1,4‐NAD(P)H2 than the wild type renalase enzyme, and/or a higher oxidation activity for 1,2‐NAD(P)H2 and 1,6‐NAD(P)H2 than the wild type renalase enzyme, wherein preferably the mutant form is recombinantly expressed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium. 26. The method of any one of claims 16‐22, wherein the renalase enzyme and/or Mung Bean Phenol Oxidase enzyme is recombinantly expressed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium. 27. The method of any one of claims 16‐22, wherein the Mung Bean Phenol Oxidase enzyme is a mutant form having a lower oxidation activity for 1,4‐NAD(P)H2 than the wild type Mung Bean Phenol Oxidase enzyme, and/or a higher oxidation activity for 1,2‐NAD(P)H2, 1,6‐NAD(P)H2 and/or [NAD(P)]2 than the wild type Mung Bean Phenol Oxidase enzyme, wherein preferably the mutant form is recombinantly expressed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium. 28. The method of any one of claims 16‐22, further comprising returning the third process stream to the cathode chamber. |
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to and the benefit of U.S. Provisional Application No. 62/308,175 filed March 14, 2016, the disclosure of w hich is incorporated herein by reference in its entirety. TECHNICAL FIELD
[0002] The present disclosure relates generally to the use of biologically mediated reactions that alter the oxidation state of compounds, and specifica lly the oxidation state of carbon atoms in a given chemical compound.
[0003] More specifically, the present disclosure relates to an improved method of utilizing the Electrochemical Bioreactor Module (EBM) in which undes ired forms of nicotinamide adenine dinucleotide cofactor which are produced during the e lectrochemical reduction of the oxidized form of the nicotinamide adenine dinucleotide cofactor are recovered for recycling through the EBM.
BACKGROUND
[0004] The use of enzymes which perform a reduction, and a re capable of reducing a provided substrate to a desired reduced product, has been wid ely reported. (Dodds et al, J. Am. Chem. Soc., (1988) 110(2), 577-583; Dodds et al, Proceedings Chir al Europe’95. London, (1995), 55‐62; A. Liese et al, Appl Microbiol Biotechnol (2007) 76:237–248; Liese, A.; 2nd ed. Enzyme Catalysis in Organic Synthesis, (2002), 3, 1419‐1459; Kula, M. R.; Kragl , U. Stereoselect. Biocatal. (2000), 839–866; Chartrain, M.; Greasham, R.; Moore, J.; Reider, P.; Robinson, D.; Buckland, B. J. Mol. Catal. B: Enzym. (2001), 11, 503–512; Patel, R. N. Enzyme Mi crob. Technol. (2002), 31, 804–826; Patel, R. N. Adv. Appl. Microbiol. (2000), 47, 33–78; Patel, R. N.; Hanson, R. L.; Banerjee, A.; Szarka, J. Am. Oi l Chem. Soc. (1997), 74, 1345–1360; Hummel, W. Adv. Biochem. Eng. Biotechnol. (1997), 58, 145– 184; Whitesides et al, Appl. Biochem. and Biotech. ( 1987) 14, 147‐197; Whitesides et al,
Biotechnology and Genetic Engineering Reviews, Vol. 6. September 1988).
[0005] To accomplish such a reduction of a provided substra te, electrons must be provided to the reaction. In biological systems, both in vivo and in vitro, these electrons, generally called “reducing equivalents”, are provided by small mole cules generally termed “cofactors”. The most common cofactor is nicotinamide adenine dinucleotide, NAD. A phosphorylated form of the cofactoralso exists, and both forms provide reducing equivalents to the enzymes that catalyze reactions requiring reducing equivalents.
[0006] The nicotinamide adenine dinucleotide cofactors exist in an oxidized state and in a reduced state, and these are respectively abbreviated as NAD(P)+ and NAD(P)H in the open literature. However, these abbreviations will be avo ided here.
[0007] Most generally, the enzymes accepting reducing equival ents from the reduced state of the nicotinamide adenine dinucleotide cofactors are termed “oxidoreductases” and are classified by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology as EC 1.n.n.n . Of particular interest are the enzyme s generally termed “dehydrogenases” or “ketoreductases” in the classes EC 1.1.n.n and EC 1.2.n.n, as well as those termed “mono‐ oxygenases” in the classes EC1.13.n.n and EC.1.14.n. n . This last group of enzymes are also termed “P450” enzymes or “CYP” enzymes.
[0008] When performing reactions catalyzed by such enzymes u sing micro‐organisms growing on a carbon source such as a carbohydrate, the reducing equivalents are generated by oxidizing a portion of the carbohydrate to CO 2 , that is, some of the carbohydrate provided t o the micro‐ organism is sacrificially oxidized in order to provid e electrons for the micro‐organism to use in various metabolic processes. While the resulting ele ctrons are desirable and useful to the microorganism, the carbon atoms sacrificed by oxidatio n to CO 2 are lost. It is also possible to provide carbon sources other than carbohydrates that are also sacrificed to produce the desired reducing equivalents. It will be clear that while such an in vivo process may perform the desired reaction catalyzed by the oxidoreductase enzyme, recov ering the desired product from the milieu of the in vivo system, e.g. a fermentation broth, c an be difficult. It is thus advantageous to utiliz e an in vitro system for performing the desired reacti on.
[0009] It is possible to isolate the needed oxidoreductase enzyme and use it to catalyze reactions in vitro. Significantly improved chemical processes could be achieved by an in vitro system which allows the use of the plethora of oxidoreductase enz ymes in processes resembling standard catalytic chemical processes. Such systems avoid the issues of recovering the desired product of the reaction from fermentation broths, and can provid e further advantages by allowing the enzyme to be used under non‐physiological conditions , and the use of a variety of methods to immobilize or otherwise contain the enzyme, and allow it to be used for extended periods of time. Such immobilization or other containment methods also allow simple and efficient recovery of the desired product of the reaction from the enzyme syst em. A further advantage of using isolated enzymes in a cell‐free system is that multiple enz ymes can be used, allowing a series of reactions to be performed.
[0010] Micro‐organisms containing useful oxidoreductases are widely known in nature and can be found quickly by simple screening (A. Zaks et al, T etrahedron (2004) 60,789–797). If a particular oxidoreductase enzyme is required, the enzyme can be readily cloned and over‐expressed in a standard industrially useful host such as S.cerevisiae or E.coli, and isolated by standard methods, and used in an in vitro system.
[0011] However, the reducing equivalents must still be provi ded to such in vitro systems. Analogous to in vivo systems, a sacrificial substrate can be provided, which is oxidized. This generates the necessary reduced state of the nicotina mide adenine dinucleotide cofactors for providing reducing equivalents for the desired reactio n.
[0012] It will be clear that the need of a sacrificial su bstrate removes at least some of the advantages of an in vivo system, since a second pro duct, the product of the oxidation of the sacrificial substrate, must now be separated from the desired product. While formate may be used sacrificial substrate, generating CO 2 as the result, this requires the use of an additional enzyme, formate dehydrogenase, which may not be conve nient.
[0013] Thus it is most desirable to provide the electrons required for the reaction, that is, the reducing equivalents required by the oxidoreductase en zyme, in a manner that does not require sacrificial substrates or additional enzymes.
[0014] If electrons could be provided from an external sour ce, that is, an electrical current, then the need to provide a sacrificial substrate to provi de electrons would be eliminated, and oxidoreductase enzymes could be used as conventional catalysts, performing reactions without the need for living cells or associated enzyme syste ms or processes.
[0015] This situation has been recognized by others, and a number of attempts to deliver electrons to biological systems by electrochemical met hods have been published.
[0016] It has been reported by Park and Zeikus in J. Bact eriol. 181:2403‐2410, 1999 that the compound called Neutral Red would undergo reversible chemical oxidoreductions with the nicotinamide adenine dinucleotide cofactor, that is, N eutral Red in its reduced form (NR red ) has a sufficiently low redox value that it will transfer e lectrons to, and thus reduce, the nicotinamide adenine dinucleotide cofactor from its oxidized state to its reduced state. In this process, the from the cathode and thus return to the reduced for m NR red , which is in turn available to again reduce the oxidized state of the nicotinamide adenine dinucleotide cofactor.
[0017] United States Patent No. 7,250,288 B2 to Zeikus et al. discusses the need for improving electrode efficiencies in electrochemical bioreactor sy stem and proposes improvements such as linking the nicotinamide adenine dinucleotide cofactor , Neutral Red, and the enzyme fumarate reductase to the electrode in order to improve elect ron transfer characteristics. While the above may improve electron transfer characteristics, it may also be advantageous to improve upon the electrochemical bioreactor system design and its use in other ways such as those described below.
[0018] The requirements for providing reducing equivalents by electrochemical methods are disclosed in PCT publication No. WO2014039767 A1, “ Electrochemical Bioreactor Module and Methods of Using the Same”. The use of isolated enzymes in conjunction with the
electrochemical reduction of the oxidized form of nicotinamide adenine dinucleotide cofactor to its reduced form, and considerations to be made, are disclosed in PCT publication No.
WO2016070168 A1, “Improved Electrochemical Bioreact or Module and Use Thereof”.
[0019] When the oxidized state of the nicotinamide adenine dinucleotide cofactor is
electrochemically reduced, several different reduced species can result. These species differ only in the position on the nicotinamide ring at which t he reduction has formally occurred. This can be at the 2‐position, the 4‐position or the 6‐posi tion on the nicotinamide ring, and these species are termed the 1,2‐dihydro‐NAD, 1,4‐dihydro‐NAD and 1,6‐dihydro‐NAD isomers respectively. (Chakraverty et al, Biochem. Biophys. Res Comm., (196 4), 15(3), 262‐268; Chakreverty et al, Jour. Biol. Chem (1969), 244(15), 4208‐4217). These are shown below in Illustration A. The carbon atoms of the nicotinamide ring are numbered in the oxidized form, with the nitrogen atom as position 1. The desired reduced product is the 1,4 ‐dihydro‐NAD isomer, commonly called β‐ NADH, and this is the biologically active form of t he co‐factor required for most dehydrogenase, ketoreductase and P450 enzymes. The other isomers, 1,2‐dihydro‐NAD and 1,6‐dihydro‐NAD do not have known useful biological activity with dehydr ogenase, ketoreductase or P450 enzymes. [0020] A fourth species, a dimer of the NAD cofactor, can also form during the electrochemical reduction of the oxidized state of nicotinamide adeni ne dinucleotide. This is termed the 4,4’‐ dimer and is also shown in Illustration A. Like t he 1,2‐dihydro‐NAD and1,6‐dihydro‐NAD forms of the cofactor, the dimer has no known useful biologic al activity with respect to oxidoreductase 3328‐3333; Biellmann et al, Tetrahedron Letters (197 8) 7, 683‐686; Kirkor et al, Eur. J. Biochem. (2000), 267, 5014‐5022).
[0021] In a system where an initial amount of the oxidized state of the nicotinamide adenine dinucleotide cofactor is electrochemically reduced for the purpose of providing reducing equivalents to an oxidoreductase enzyme, the desired, biologically active 1,4‐dihydro‐NAD isomer will be formed and will provide reducing equivalents to the oxidoreductase enzyme (or enzymes) in the system, and be oxidized back to the oxidized nicotinamide adenine dinucleotide cofactor, ready for another cycle of electrochemical reduction. However, the two non‐biologically active isomers, the 1,2‐dihydro‐NAD and the 1,6‐dihydro NAD, as well as the 4,4’‐dimer initially produced by the electrochemical reduction of the oxid ized nicotinamide adenine dinucleotide cofactor will not be consumed and will simply remain in the system. The oxidized nicotinamide adenine dinucleotide cofactor resulting from the produ ctive delivery of reducing equivalents to the oxidoreductase enzyme will be again reduced to g ive a mixture of the three isomers, plus the dimer, as in the previous reduction process. In this cyclic manner, the amount of oxidized continually, and after a short period of time, essen tially none is left. This problem was recognized four decades ago (Whitesides et al, Appl. Biochem. a nd Biotech. (1987) 14, 147‐197; Whitesides et al, Biotechnology and Genetic Engineering Reviews, Vol . 6. September 1988).
[0022] Thus, it is useful to remove the undesired 1,2‐dih ydro‐NAD and the 1,6‐dihydro‐NAD isomers as well as the 4,4’‐dimer should they be produced during the electrochemical reduction of oxidized nicotinamide adenine dinucleotide cofactor to the desired 1,4‐dihydro‐NAD species, (i.e. ^‐NADH). Further, it is useful to recover the 1, 2‐dihydro‐NAD and the 1,6‐dihydro‐NAD isomers a s either as the desired 1,4‐dihdro‐NAD isomer, or a s the initial oxidized state of the nicotinamide adenine dinucleotide cofactor.
[0023] Provided herein are methods and systems for achieving the above objectives. Other objectives, features, and advantages of the present d isclosure will be apparent on review of the specification and claims.
SUMMARY
[0024] The present disclosure, in one aspect, provides a sy stem for electrochemically generating NAD(P)H 2 reducing equivalents, the system comprising:
(a) an electrochemical cell comprising an anode contained in an anode chamber and a cathode contained in a cathode chamber;
(b) a first process stream containing NAD(P), passing thr ough the cathode chamber and continuously in contact with the cathode from which electrons are transferred to the NAD(P) to produce a second process stream containing reduced species 1,4‐NAD(P)H 2 , 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 , and [NAD(P)] 2 , while optionally producing hydrogen; (c) a substrate of an oxidoreductase or P450 enzyme, whi ch, when contacted with the second process stream in the presence of the oxidore ductase or P450 enzyme, is transformed to a product while concomitantly consuming the 1,4‐NAD(P)H 2 in the second process stream and producing a first recovered NAD(P) and a third process stream; and
(d) at least one of a renalase enzyme, a Mung Bean Phe nol Oxidase enzyme and
illumination at a wavelength of about 254 nm or exc eeding about 320 nm, which, when contacted with the third process stream, convert at least one of the 1,2‐NAD(P)H 2 , 1,6‐ NAD(P)H 2 , and [NAD(P)] 2 therein to a second and optionally a third rec overed NAD(P). [0025] In some embodiments, the system includes the renalase enzyme for converting the 1,2‐ NAD(P)H 2 and 1,6‐NAD(P)H 2 into the second recovered NAD(P).
[0026] In some embodiments, the system includes the Mung Be an Phenol Oxidase enzyme for converting the 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 , and/or [NAD(P)] 2 into the second recovered NAD(P) and/or the third recovered NAD(P).
[0027] In some embodiments, the system includes the illumina tion for converting the [NAD(P)] 2 into NAD(P).
[0028] In some embodiments, the system includes the renalase enzyme and the Mung Bean Phenol Oxidase enzyme. For example, the third proce ss stream can be contacted with the renalase enzyme resulting in conversion of the 1,2‐ NAD(P)H 2 and 1,6‐NAD(P)H 2 into the second recovered NAD(P) and a fourth process stream, wherein the fourth process stream can be contacted with the Mung Bean Phenol Oxidase enzyme t o convert the [NAD(P)] 2 therein to the third recovered NAD(P).
[0029] In some embodiments, the system includes the renalase enzyme and the illumination. For example, the third process stream can be contacted w ith the renalase enzyme resulting in conversion of the 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 into the second recovered NAD(P) and a fourth process stream, wherein the fourth process stream can be contacted with the illumination to convert the [NAD(P)] 2 therein to the third recovered NAD(P).
[0030] In some embodiments, the system includes the Mung Be an Phenol Oxidase enzyme and the illumination. For example, the third process st ream can be contacted with the Mung Bean Phenol Oxidase enzyme resulting in conversion of the 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 into the second recovered NAD(P) and a fourth process stream, wherein the fourth process stream can be contacted with the illumination to convert the [NAD(P )] 2 therein to the third recovered NAD(P). [0031] In some embodiments, the system can include, in one or more of the process streams, an electron transfer mediator (ETM) capable of transferri ng electrons to NAD(P). The system can also include catalase for decomposing hydrogen peroxid e produced by the renalase enzyme, the Mung Bean Phenol Oxidase enzyme, and/or the illuminat ion.
[0032] Another aspect relates to a method for electrochemica lly generating NAD(P)H 2 reducing equivalents, the method comprising:
(a) providing an electrochemical cell comprising an anode contained in an anode chamber and a cathode contained in a cathode chamber; and is continuously in contact with the cathode from which electrons are transferred to the NAD(P) to produce a second process stream co ntaining reduced species 1,4‐ NAD(P)H 2 , 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 , and [NAD(P)] 2 , while optionally producing hydrogen;
(c) contacting the second process stream with a substrate of an oxidoreductase or P450 enzyme such that the substrate, in the presence of the oxidoreductase or P450 enzyme, is transformed to a product while concomitant ly consuming the 1,4‐NAD(P)H 2 in the second process stream and producing a first recovered NAD(P) and a third process stream; and
(d) contacting the third process stream with at least on e of a renalase enzyme, a Mung Bean Phenol Oxidase enzyme and illumination at a wav elength of about 254 nm or exceeding about 320 nm, thereby converting at least one of the 1,2‐NAD(P)H 2 , 1,6‐ NAD(P)H 2 , and [NAD(P)] 2 therein to a second recovered NAD(P) and option ally a third recovered NAD(P).
[0033] The method in some embodiments can further include c ontacting the third process stream with the renalase enzyme for converting the 1,2‐NAD (P)H 2 and 1,6‐NAD(P)H 2 into the second recovered NAD(P).
[0034] In some embodiments, the method can further include contacting the third process stream with the Mung Bean Phenol Oxidase enzyme for convert ing the 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 , and/or [NAD(P)] 2 into the second recovered NAD(P) and/or the t hird recovered NAD(P).
[0035] In some embodiments, the method can further include contacting the third process stream with the illumination for converting the [NAD(P)] 2 into NAD(P).
[0036] In some embodiments, the method can further include contacting the third process stream with the renalase enzyme resulting in conversion of the 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 therein into the second recovered NAD(P) and a fourth proces s stream, and further comprising contacting the fourth process stream with the Mung Bean Phenol Oxidase enzyme to convert the [NAD(P)] 2 therein to the third recovered NAD(P).
[0037] In some embodiments, the method can further include contacting the third process stream with the renalase enzyme resulting in conversion of the 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 therein into the second recovered NAD(P) and a fourth proces s stream, and further comprising contacting the fourth process stream with the illumination to c onvert the [NAD(P)] 2 therein to the third [0038] In some embodiments, the method can further include contacting the third process stream with the Mung Bean Phenol Oxidase enzyme resulting i n conversion of the 1,2‐NAD(P)H 2 and 1,6‐ NAD(P)H 2 therein into the second recovered NAD(P) and a fourth process stream, and further comprising contacting the fourth process stream with the illumination to convert the [NAD(P)] 2 therein to the third recovered NAD(P).
[0039] In some embodiments, the method can further include providing, in one or more of the process streams, an electron transfer mediator (ETM) capable of transferring electrons to NAD(P). [0040] In some embodiments, the method can further include providing catalase for decomposing hydrogen peroxide produced by the renalase enzyme, th e Mung Bean Phenol Oxidase enzyme, and/or the illumination.
[0041] In some embodiments, the method can further include returning the third process stream to the cathode chamber.
[0042] In any of the systems and methods disclosed herein, the renalase enzyme and/or Mung Bean Phenol Oxidase enzyme may be recombinantly expre ssed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium gl utamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium.
[0043] The renalase enzyme in certain embodiments can be a mutant form (naturally occurring or genetically engineered) having a lower oxidation activ ity for 1,4‐NAD(P)H 2 than the wild type renalase enzyme, and/or a higher oxidation activity f or 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 than the wild type renalase enzyme. In some examples, the m utant form can be recombinantly expressed from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium.
[0044] The Mung Bean Phenol Oxidase enzyme can be a mutant form (naturally occurring or genetically engineered) having a lower oxidation activ ity for 1,4‐NAD(P)H 2 than the wild type Mung Bean Phenol Oxidase enzyme, and/or a higher oxi dation activity for 1,2‐NAD(P)H 2 , 1,6‐ NAD(P)H 2 and/or [NAD(P)] 2 than the wild type Mung Bean Phenol Oxidase enzyme. In some embodiments, the mutant form is recombinantly expresse d from microorganisms such as Escherichia coli, Bacillus subtilis, Corynebacterium gl utamicum, Saccharomyces cerevisiae, Pichia pastoris, and Bacillus megaterium.
[0045] Also provided herein is a process for providing elec trochemically generated reducing power via the reduction of an oxidized form of phos phorylated or non‐phosphorylated forms of catalyzing a transformation of a substrate molecule.
[0046] The process can include steps for preventing the los s of cofactor material to reduced forms that are not used by the redox enzyme system for t he purpose of catalyzing a transformation of a substrate molecule.
[0047] The process can include the electrochemical reduction of the oxidized state of
nicotinamide adenine dinucleotide cofactor to give a mixture of the desired 1,4‐dihydro‐NAD species with the undesired 1,2‐dihydro‐NAD and 1,6 ‐dihydro‐NAD species, and the undesired 4,4’‐ dimer. The desired 1,4‐dihydro‐NAD species is co nverted back to the oxidized form of the nicotinamide adenine dinucleotide cofactor in the cour se of providing reducing equivalents to the oxidoreductase enzyme which is present to catalyze th e transformation of the substrate molecule. The remaining mixture of the 1,2‐dihydro‐NAD and 1,6‐dihydro‐NAD species, the 4,4’‐dimer, together with the oxidized state of the nicotinamide adenine dinucleotide cofactor is acted upon by the renalase enzyme. This enzyme oxidizes the 1 ,2‐dihydro‐NAD and 1,6‐dihydro‐NAD species back to the oxidized state of the nicotinamide adeni ne dinucleotide cofactor in the presence of oxygen, producing hydrogen peroxide in the process. The Mung Bean enzyme oxidizes the 4,4’‐ dimer back to two molecules of the oxidized state o f the nicotinamide adenine dinucleotide cofactor, also by using oxygen and generating hydroge n peroxide. Thus all species originally produced by the electrochemical reduction of the oxid ized form of the nicotinamide adenine dinucleotide cofactor, both desired and undesired, are returned to the original oxidized state of the nicotinamide adenine dinucleotide cofactor and no cofactor material is lost. The 4,4’‐dimer is photo‐active, and can be returned to the oxidized state of the nicotinamide adenine dinucleotide cofactor species by irradiation with the appropriate wavelength; this may be performed in place of, or in conjunction with, the Mung Bean oxidase.
BRIEF DESCRIPTION OF THE DRAWINGS
[0048] FIG. 1 illustrates, in a schematic, the utilization of renal ase, Mung Bean Phenol Oxidase, and optionally illumination to recover and recycle co factor material from the mixture of the desired 1,4‐dihydro‐NAD species plus the undesired 1,2‐dihydro‐NAD and 1,6‐dihydro‐NAD species and the undesired 4,4’‐dimer which are fo rmed via the electrochemical reduction of the oxidized state of nicotinamide adenine dinucleotide co factor. In Figure 1, the 1,2‐dihydro‐NAD, 1,4‐dihydro‐NAD and the 1,6‐dihydro‐NAD species of the nictotinamide adenine dinucleotide dimer is named [NAD(P)] 2 and the oxidized form is named NAD(P).
DETAILED DESCRIPTION
[0049] The current literature most commonly uses the descrip tor “NAD(P)+” to indicate the oxidized state of the phosphorylated and non‐phospho rylated forms of the nicotinamide adenine dinucleotide, and “NAD(P)H” to indicate the reduce d state. The reduced state of the
phosphorylated and non‐phosphorylated forms of the nicotinamide adenine dinucleotide are produced by adding two electrons and two protons to the oxidized state. This common nomenclature is misleading, as plain reading of the common descriptors “NAD(P)+” and “NAD(P)H” show the oxidized state of the phosphor ylated and non‐phosphorylated forms of the nicotinamide adenine dinucleotide to be a positively charged species “NAD(P)+”, while the reduced state of the phosphorylated and non‐phosphor ylated forms of the nicotinamide adenine dinucleotide, “NAD(P)H”, is shown to have only on e hydrogen added relative to the oxidized state. Neither of these representations is true. The oxidi zed state of the phosphorylated and non‐ phosphorylated forms of the nicotinamide adenine dinuc leotide is a neutral molecule in which the nitrogen of the nicotinamide ring bears a formal pos itive charge with is balanced by a negative charge one of the deprotonated phosphate linkages, th us forming an internal salt or zwitterion. The reduced state of the phosphorylated and non‐pho sphorylated forms of the nicotinamide adenine dinucleotide has formally accepted a hydrogen molecule, H 2 , relative to the oxidized state, and this achieved by two one‐electron transf ers balanced by two protons. This is shown formally by the following balanced chemical reaction:
NAD(P) + 2H + + 2e- → NAD(P)H 2
[0050] The published molecular weights of the oxidized and reduced states of the non‐
phosphorylated form of the nicotinamide adenine din ucleotide indicate the reality of this reaction clearly, the molecular weights being 663.43 Da and 6 65.44 Da respectively and the difference being the molecular weight of a single hydrogen mole cule, H2.
[0051] In the present disclosure, the descriptor “NAD(P)” indicates the oxidized state of the phosphorylated and non‐phosphorylated forms of the n icotinamide adenine dinucleotide, while the descriptor “NAD(P)H 2 ” indicates the reduced state of the phosphor ylated and non‐
phosphorylated forms of the nicotinamide adenine di nucleotide. Further, the descriptors “1,2‐ NAD(P)H 2 ”,“1,4‐NAD(P)H 2 ”and“1,6‐NAD(P)H 2 ”are used to indicate the three stereoisomers of been formally added across the 1,2‐, 1,4‐ and 1, 6‐positions of the nicotinamide ring. It will be clear to those skilled in the art that 1,4‐NAD(P)H 2 is commonly called β‐NADH, and is the stereoisomer of the reduced state of the phosphorylat ed and non‐phosphorylated forms of nicotinamide adenine dinucleotide which is active with oxidoreductase and P450 enzymes.
[0052] In the present disclosure, the 4,4’‐dimer that is also known to form as a consequence of a one‐electron transfer to NAD(P) is indicated by the descriptor “[NAD(P)] 2 ”.
[0053] The present disclosure, in some embodiments, is direc ted to an improved version and improved use of the “Electrochemical Bioreactor Modu le” (EBM) previously described in PCT Patent Application Publication Nos. WO2014039767 A1 an d WO2016070168 A1, which are incorporated herein by reference in its entirety.
[0054] An EBM can include one or more of the following co mponents:
a. an anode contained in an anode chamber and a cathod e contained in a cathode
chamber;
b. deionized water in the anode chamber in contact with the anode;
c. a proton permeable membrane that separates the anode and cathode chambers; d. a liquid phase in the cathode chamber continuously i n contact with the cathode, said liquid phase optionally comprising an electron transfe r mediator (ETM) capable of transferring reducing equivalents to a redox enzyme s ystem, said redox enzyme system comprising a redox enzyme and a cofactor;
e. a process stream containing a substrate to be tran sformed via catalysis by the redox enzyme system into a desired product;
f. a membrane located between the cathode and the pro cess stream, said membrane capable of preventing the optional ETM and the redox enzyme system from significantly entering into the process stream; and
g. an external power source providing a voltage between the anode and the cathode. [0055] In certain embodiments, an improved EBM or process u sing the EBM of the present disclosure can include the enzyme renalase, the Mung Bean Phenol Oxidase, and illumination to recover the undesired forms of cofactor that result from electrochemical reduction of NAD(P). [0056] The enzyme renalase (Moran et al, J. Am. Chem. Soc. (2013), 135, 13980−13987; Moran, G.R., Biochimica et Biophysica Acta 1864 (2016) 177 186), is capable of oxidizing the 1,2‐dihydro‐ NAD and 1,6‐dihydro‐NAD species back to the oxidi zed state of the nicotinamide adenine [0057] Another enzyme isolated from Mung Bean and currently termed “Mung Bean Phenol Oxidase” (Fricks et al, Arch. Biochem. Biophys, (19 73)169, 837‐841). This enzyme oxidizes the 4,4’‐dimer, [NAD(P)] 2 , that can be formed under electrochemically reducing conditions back to the oxidized state of nicotinamide adenine dinucleotid e. This enzyme has also been reported as having the same activity as renalase and being able to oxidize the 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 species back to the oxidized state of nicotinamide a denine dinucleotide as well (Kono et al, Bull. Agr. Chem. Soc. Japan, (1958) 22(6) 404‐410).
[0058] It has also recently been shown that illumination of electrochemically prepared 4,4’‐dimer at about 254 nm or at wavelengths exceeding 320 nm leads to regeneration of the oxidized state of nicotinamide adenine dinucleotide. (Czochralska et al, (1980) Arch. Biochem. Biophys. 199, 497; Czochralska et al, (1990) Photochem. Photobiol. 51, 4 01‐410).
[0059] Thus it is useful to incorporate the renalase and M ung Bean Phenol Oxidase enzymes, and the illumination of the [NAD(P)] 2 dimer of nicotinamide adenine dinucleotide in a process for the recovery of cofactor material, and for the prevention of the loss of cofactor material to forms which are not used by oxidoreductase enzymes such as dehydrogenases, ketoreductases or P450 enzymes.
[0060] In one embodiment, the present disclosure provides a process that comprises:
a) a process stream containing NAD(P) which is passed t hrough the cathode chamber of an electrochemical cell such that the desired, bi ologically active 1,4‐NAD(P)H 2 is produced, together with the undesired 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 and [NAD(P)] 2 species;
b) providing a substrate molecule that is to be transfo rmed via the catalytic reaction of an oxidoreductase enzyme, such as a dehydrogenase or ketoreductase, or a P450 enzyme, to produce a desired product molecule;
c) contacting the process stream containing both the des ired and undesired cofactor species with an oxidoreductase, such as a dehydrogena se or ketoreductase, or a P450 enzyme, capable of using the reducing equivalent s presented by the 1,4‐ NAD(P)H 2 to perform a desired reaction in which a sub strate molecule is transformed to a desired product molecule, while the 1,4‐NAD(P)H 2 is concomitantly transformed back to NAD(P);
d) recovering the cofactor material from the undesired , leaving the undesired 1,2‐ contacting the process stream with the renalase enzym e in the presence of oxygen for a sufficient time that the renalase enzyme can catalyze the oxidation of the undesired 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 species that may be present in the process stream to the oxidized form of the cofactor, NAD(P);
e) recovering the cofactor material from the undesired a nd [NAD(P)] 2 species present in the process stream by contacting the process stre am with Mung Bean Phenol Oxidase in the presence of oxygen for a sufficient time that the Mung Bean Phenol Oxidase enzyme can catalyze the oxidation and any [N AD(P)] 2 present in the process stream to the oxidized form of the cofactor NAD(P);
f) returning the process stream now containing all of t he original nicotinamide
adenine dinucleotide as the oxidized form NAD(P) to the cathode chamber of the electrochemical cell for reduction.
[0061] In one embodiment, a process stream containing NAD(P) is passed through the cathode chamber of the electrochemical cell such that 1,2‐N AD(P)H 2 , 1,4‐NAD(P)H 2 , 1,6‐NAD(P)H 2 and the 4,4’‐dimer, [NAD(P)] 2 are produced. The process stream containing the mixture of NAD(P)H 2 species is then contacted with an oxidoreductase enzy me (e.g. a dehydrogenase, ketoreductase, or P450 enzyme) capable of using the reducing equiva lents from the 1,4‐NAD(P)H 2 to perform a desired reaction in which a substrate molecule is tr ansformed to a desired product molecule, while the 1,4‐NAD(P)H 2 is concomitantly transformed back to NAD(P). The enzyme utilizing the 1,4‐NAD(P)H 2 may be present in the process stream, immobil ized on beads or other suitable material and held in a packed column, or contained by a membrane as described in PCT Patent Application Publication No. WO2016070168 A1, which is incorporated herein by reference in its entirety.
[0062] The process stream, after contacting the oxidoreductas e enzyme and after the 1,4‐ NAD(P)H 2 has been transformed back to NAD(P), is then contacted with the renalase enzyme. In the presence of oxygen, the renalase enzyme oxidizes the undesired 1,2‐NAD(P)H 2 and 1,6‐ NAD(P)H 2 species that may be present back to NAD(P) w ith the concomitant production of hydrogen peroxide. An enzyme such as catalase, or a suitable inorganic catalyst (e.g. MnO 2 ), may be present to decompose the hydrogen peroxide and th us prevent deleterious effects by the hydrogen peroxide.
[0063] The renalase enzyme may be immobilized on beads or a suitable support, immobilized on, standard methods known to those skilled in the art of enzymatic reactions. As the renalase enzyme has some activity towards the desirable 1,4‐ NAD(P)H 2 species and will oxidize this back to NAD(P), it is better if the renalase enzyme is not freely circulating in the process stream, and that the process stream is contacted with the renalase af ter the 1,4‐NAD(P)H 2 present in the process stream has been consumed by the oxidoreductase enzyme , and has been used to perform a useful reaction on a substrate.
[0064] The process stream is then brought into contact with the Mung Bean Phenol oxidase in the presence of oxygen and any undesired 4,4’‐dimer, [NAD(P)] 2 , present is thus oxidized back to NAD(P) with the concomitant production of hydrogen pe roxide. The Mung Bean Phenol Oxidase may be immobilized on beads or a suitable support, immobilized on, or contained by, a membrane or kept from freely circulating in the process strea m by other standard methods known to those skilled in the art of enzymatic reactions. As the Mung Bean Phenol Oxidase enzyme has some activity towards the desirable 1,4‐NAD(P)H 2 species and will oxidize this back to NAD(P), it is better if the Mung Bean Phenol Oxidase enzyme is no t freely circulating in the process stream, and that the process stream is contacted with the M ung Bean Phenol Oxidase after the desirable 1,4‐NAD(P)H 2 present in the process stream has been consum ed by the oxidoreductase enzyme. [0065] In another embodiment, the process stream, after cont acting the oxidoreductase enzyme, may be contacted first with the Mung Bean Phenol Ox idase enzyme for oxidation of the undesired [NAD(P)] 2 back to NAD(P), and then subsequently with th e renalase enzyme for oxidation of the undesired 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 species back to NAD(P).
[0066] In another embodiment, the process stream, after cont acting the oxidoreductase enzyme may be contacted with the renalase enzyme and subseq uently irradiated with light at 254 nm or at wavelengths exceeding 320 nm for decomposing the undesired [NAD(P)] 2 dimer back to NAD(P).
[0067] In another embodiment, the process stream, after cont acting the oxidoreductase enzyme, may be contacted not only with the Mung Bean Phenol Oxidase enzyme for oxidation of the undesired [NAD(P)] 2 dimer back to NAD(P), and also for oxidation of the undesired 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 species to NAD(P) as the Mung Bean Phenol Ox idase enzyme is known to have activity for the oxidation of the undesired 1,2‐NAD (P)H 2 and 1,6‐NAD(P)H 2 species back to NAD(P). [0068] It will be clear to those skilled in the art that the order of the steps in the process for recovering cofactor material as NAD(P) from the undes ired 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 , and Mung Bean Phenol Oxidase or illuminating it with lig ht at 254 nm or at wavelengths exceeding 320 nm may be done in any order or combination.
[0069] It will be clear to those skilled in the art that the Mung Bean Phenol Oxidase and the renalase enzymes may be engineered by known methods to allow greater stability and therefore lifetime in the process, or to allow greater activit y, or to allow increased ease and efficiency of immobilization, or to allow the more efficient expres sion and isolation of these enzymes.
[0070] It will be equally clear to those skilled in the a rt of enzymatic reactions that the Mung Bean Phenol Oxidase enzyme may be engineered to allow inc reased activity for the oxidation of the undesired 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 , species to NAD(P), in addition to its activi ty for oxidizing the undesired [NAD(P)] 2 dimer to NAD(P).
[0071] In certain embodiments, the renalase enzyme and the Mung Bean Phenol Oxidase enzyme may be engineered to have insignificant (e.g., lower than wide‐type) activity on the desirable 1,4‐ NAD(P)H 2 species, but useful (e.g., higher than wide‐ type) activity on the undesired 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 , and [NAD(P)] 2 species resulting from the electrochemical redu ction of NAD(P). These enzymes may then be circulated with the proces s stream, or the enzymes may be immobilized or otherwise contained and contacted with the process stream in any order that is convenient, including prior to contacting the process stream with the oxidoreductase or P450 enzyme which is acting upon the provided substrate.
EXAMPLES
Example I
[0072] This example describes an exemplary process in which the undesired 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 isomers are re‐oxidized to NAD(P) by renalas e, and the undesired [NAD(P)] 2 dimer is oxidatively cleaved to NAD(P) by Mung Bean Phenol Ox idase.
[0073] To demonstrate the process described in the present disclosure, Mung Bean Phenol Oxidase is extracted from Mung Bean seedlings as des cribed by Burnett and Underwood
(Biochem. 7(10), 3328‐3333, 1968). The gene hum an renalase isoform 1 is cloned onto a plasmid for expression in E.coli, including codon optimization . The sequence of the renalase is revealed by Pandini et al. (Protein Expression and Purification 7 2 (2010) 244–253). The enzyme is expressed as an inclusion body, the inclusion bodies collected and refolded as described by Padini et al (Protein Expression and Purification 72 (2010) 244–2 53). The renalase enzyme (MW = 35 KDa) is between the inlet and the outlet that is of a suff iciently low molecular weight cutoff to prevent the renalase from passing through it, but also havin g a sufficiently high molecular weight cutoff that all species of the nicotinamide adenine dinucleo tide present in the process stream can pass through it. A membrane with a molecular weight cut off of 10KDa is used. In a similar manner, the Mung Bean Phenol Oxidase enzyme is held in a separa te container with an inlet and an outlet and that also includes a membrane between the inlet and the outlet that is permeable, having a sufficiently low molecular weight cutoff that the Mun g Bean Phenol Oxidase cannot pass through it, but a sufficiently high molecular weight cutoff that all species of the nicotinamide adenine dinucleotide present in the process stream can pass through it. A membrane with a molecular weight cutoff of 10KDa is used. To allow the use of an oxidoreductase enzyme that demonstrates the presence of useful amounts of the desired 1,4‐ NAD(P)H 2 cofactor, the membrane system described in published PCT Patent Application WO201607 0168 A1 published 6 May 2016, and which is also described in United States Patent Nos. 4,705,704 and 5,077,217 for the purpose of containing an enzyme. The enzyme Horse Liver Alcoho l Dehydrogenase is used, with
cyclohexanone or benzaldehyde used as the substrate for the alcohol dehydrogenase enzyme. A process stream containing 1 to 2 mM of NAD(P) is p repared and buffered to a pH suitable for all three enzymes; a pH of 7.5 is used. The process stream containing the NAD(P) is pumped through the cathode chamber of the electrochemical cell, whic h is energized at approximately 2 volts, and the NAD(P) cofactor is reduced to give the desired 1,4‐NAD(P)H 2 form of the cofactor, and also the undesired 1,2‐NAD(P)H 2 , 1,6‐NAD(P)H 2 and 4,4’‐dimer, [NAD(P)] 2 forms of the cofactor. Exiting the cathode chamber, the NAD(P) process stream goes through the membrane system containing the alcohol dehydrogenase, where the desired 1,4‐NAD (P)H 2 form of the cofactor provides reducing equivalents to the alcohol dehydrogenase, the cyclohexanone or benzaldehyde substrate is reduced to cyclohexanol or benzyl alcohol product respectively, thus consuming the 1,4‐ NAD(P)H 2 form of the cofactor and producing the oxidiz ed form NAD(P). The process stream then proceeds to the inlet of the container holding the renalase enzyme. The process stream mixes with the renalase enzyme and the renalase enzyme oxi dizes the undesired 1,2‐NAD(P)H 2 and 1,6‐ NAD(P)H 2 forms of the cofactor to NAD(P). The proces s stream then passes through the membrane but the renalase enzyme is held in its con tainer by the membrane. After passing through the membrane the process stream proceeds to the outlet of the container holding the renalase enzyme. From here the process stream passe s to the inlet of the container holding the Oxidase enzyme and the enzyme oxidizes the undesired 4,4’dimer, [NAD(P)] 2 form of the cofactor to NAD(P). The process stream then passes through the membrane but the Mung Bean Phenol Oxidase enzyme is held in its container by the memb rane. The process stream exits through the outlet of the container holding the Mung Bean Phenol Oxidase enzyme, and now contains only NAD(P)as the other forms of the cofactor have all b een oxidized by the alcohol dehydrogenase, the renalase and the Mung Bean Phenol Oxidase enzyme s. The process stream is returned to the pump, and sent into the cathode chamber to repeat t he cycle.
Example II
[0074] This example describes an exemplary process in which the undesired 1,2‐NAD(P)H 2 and 1,6‐NAD(P)H 2 isomers are re‐oxidized to NAD(P) by renalas e, and the undesired [NAD(P)] 2 dimer is oxidatively cleaved to NAD(P) by illumination with UV light at 254 nm.
[0075] The process described in Example I is used, exceptin g the container holding the Mung Bean Phenol Oxidase is replaced by a quartz tube through which the process stream can flow unimpeded by any membrane. Outside the quartz tube an ultraviolet lamp is present, and illuminates the tube with light at 254 nm which pas ses through the quartz and photolytically cleaves the 4,4‐dimer of the cofactor, [NAD(P)] 2 , to NAD(P). Upon exiting the quartz tube, t he process stream is returned to the pump, and sent in to the cathode chamber to repeat the cycle.
EQUIVALENTS
[0076] The present disclosure provides among other things no vel methods and devices for providing reducing equivalents to biological systems. While specific embodiments of the subject disclosure have been discussed, the above specificatio n is illustrative and not restrictive. Many variations of the disclosure will become apparent to those skilled in the art upon review of this specification. The full scope of the disclosure sho uld be determined by reference to the claims, along with their full scope of equivalents, and the specification, along with such variations.
INCORPORATION BY REFERENCE
[0077] All publications, patents and patent applications cite d above are incorporated by reference herein in their entirety for all purposes to the sa me extent as if each individual publication or patent application were specifically indicated to be so incorporated by reference.