Title:
INSULIN-PRODUCING CELLS
Document Type and Number:
WIPO Patent Application WO/2020/059892
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a novel method for making it possible to efficiently induce/produce endocrine cells from pluripotent stem cells. Insulin-producing cells including cells that are insulin-positive and NKX6.1-positive in a ratio of at least 30%, and cells that are insulin-positive and NKX6.1-negative in a ratio of more than 15%.
Inventors:
ITO RYO (JP)
YAMAZOE NORIKO (JP)
HIYOSHI HIDEYUKI (JP)
MOCHIDA TAISUKE (JP)
UENO HIKARU (JP)
SAKUMA KENSUKE (JP)
YAMAURA JUNJI (JP)
MATSUMOTO HIROKAZU (JP)
TOYODA TARO (JP)
KONAGAYA SHUHEI (JP)
YAMAZOE NORIKO (JP)
HIYOSHI HIDEYUKI (JP)
MOCHIDA TAISUKE (JP)
UENO HIKARU (JP)
SAKUMA KENSUKE (JP)
YAMAURA JUNJI (JP)
MATSUMOTO HIROKAZU (JP)
TOYODA TARO (JP)
KONAGAYA SHUHEI (JP)
Application Number:
PCT/JP2019/037725
Publication Date:
March 26, 2020
Filing Date:
September 18, 2019
Export Citation:
Assignee:
TAKEDA PHARMACEUTICALS CO (JP)
UNIV KYOTO (JP)
UNIV KYOTO (JP)
International Classes:
C12N5/071; A61K35/12; A61P3/10; A61P5/50
Domestic Patent References:
| WO2019208505A1 | 2019-10-31 | |||
| WO2009012428A2 | 2009-01-22 | |||
| WO2016021734A1 | 2016-02-11 | |||
| WO2007069666A1 | 2007-06-21 | |||
| WO2008118220A2 | 2008-10-02 | |||
| WO2008124133A1 | 2008-10-16 | |||
| WO2008151058A2 | 2008-12-11 | |||
| WO2009006930A1 | 2009-01-15 | |||
| WO2009006997A1 | 2009-01-15 | |||
| WO2009007852A2 | 2009-01-15 | |||
| WO2010032242A1 | 2010-03-25 | |||
| WO2011154941A2 | 2011-12-15 |
Foreign References:
| JP2018175465A | 2018-11-15 | |||
| JP2008307007A | 2008-12-25 | |||
| JP2008283972A | 2008-11-27 | |||
| US20080236610A1 | 2008-10-02 | |||
| US20090047263A1 | 2009-02-19 | |||
| US77394407A | 2007-07-05 |
Other References:
REZANIA, ALIREZA ET AL.: "Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells", NATURE BIOTECHNOLOGY, vol. 32, no. 11, 2014, pages 1121 - 1133, XP055200937, DOI: 10.1038/nbt.3033
PAGLIUCA, F. W. ET AL.: "Generation of Functional Human Pancreatic p Cells In Vitro", CELL, vol. 159, 9 October 2014 (2014-10-09), pages 428 - 439, XP029073423
YAMASHITA-SUGAHARA, YZUMI ET AL.: "An inhibitor of fibroblast growth factor receptor-1 (FGFRl) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors", SCIENTIFIC REPORTS, vol. 6, 27 October 2016 (2016-10-27), pages 35908, XP055646902
RICHARDSON, THOMAS ET AL.: "Alginate Encapsulation of Human Embryonic Stem Cells to Enhance Directed Differentiation to Pancreatic Islet-Like Cells", TISSUE ENGINEERING: PART A, vol. 20, no. 23, 2014, pages 3198 - 3211, XP055637763, DOI: 10.1089/ten.tea.2013.0659
REZANIA, ALIREZA ET AL.: "Enrichment of Human Embryonic Stem Cell -Derived NKX6.1-Expressing Pancreatic Progenitor Cells Accelerates the Maturation of Insulin-Secreting Cells In Vivo", STEM CELL, vol. 31, 2013, pages 2432 - 2442, XP055190599, DOI: 10.1002/stem.1489
ROBERT, THOMAS ET AL.: "Functional Beta Cell Mass from Device-Encapsulated hESC-Derived Pancreatic Endoderm Achieving Metabolic Control", STEM CELL REPORTS, vol. 10, 13 March 2018 (2018-03-13), pages 739 - 750, XP055693918
BRUIN, J. E. ET AL.: "Accelerating the in vivo maturation of macro-encapsulated human embryonic stem cell derived-pancreatic progenitor cells by manipulating NKX6. 1 expression in culture", DIABETOLOGIA, vol. 55, 2012, pages S178 - S179, XP009527010, ISSN: 0012-186X
REZANIA A. ET AL., NAT BIOTECHNOL., vol. 32, 2014, pages 1121 - 33
FELICIA W. PAGLIUCA ET AL., CELL, vol. 159, no. 2, 9 October 2014 (2014-10-09), pages 428 - 439
ROBERT T ET AL., STEM CELL REPORTS., vol. 10, no. 3, 13 March 2018 (2018-03-13), pages 739 - 750
TAKAHASHI KYAMANAKA S., CELL, vol. 126, 2006, pages 663 - 676
TAKAHASHI KYAMANAKA S. ET AL., CELL, vol. 131, 2007, pages 861 - 872
OKITA, K.ICHISAKA, T.YAMANAKA, S., NATURE, vol. 451, 2007, pages 141 - 146
HUANGFU D.MELTON, DA. ET AL., NATURE BIOTECHNOLOGY, vol. 26, no. 7, 2008, pages 795 - 797
OKITA K ET AL., NAT. METHODS, vol. 8, no. 5, May 2011 (2011-05-01), pages 409 - 12
OKITA K ET AL., STEM CELLS, vol. 31, no. 3, pages 458 - 66
YU J.THOMSON JA. ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
SHI Y.DING S. ET AL., CELL STEM CELL, vol. 3, no. 5, 2008, pages 568 - 574
KIM JB.SCHOLER HR. ET AL., NATURE, vol. 454, 2008, pages 646 - 650
TOYODA ET AL., STEM CELL RESEARCH, vol. 14, 2015, pages 185 - 197
NATURE BIOTECHNOLOGY, vol. 32, 2014, pages 1121 - 1133
See also references of EP 3854869A4
PAGLIUCA, F. W. ET AL.: "Generation of Functional Human Pancreatic p Cells In Vitro", CELL, vol. 159, 9 October 2014 (2014-10-09), pages 428 - 439, XP029073423
YAMASHITA-SUGAHARA, YZUMI ET AL.: "An inhibitor of fibroblast growth factor receptor-1 (FGFRl) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors", SCIENTIFIC REPORTS, vol. 6, 27 October 2016 (2016-10-27), pages 35908, XP055646902
RICHARDSON, THOMAS ET AL.: "Alginate Encapsulation of Human Embryonic Stem Cells to Enhance Directed Differentiation to Pancreatic Islet-Like Cells", TISSUE ENGINEERING: PART A, vol. 20, no. 23, 2014, pages 3198 - 3211, XP055637763, DOI: 10.1089/ten.tea.2013.0659
REZANIA, ALIREZA ET AL.: "Enrichment of Human Embryonic Stem Cell -Derived NKX6.1-Expressing Pancreatic Progenitor Cells Accelerates the Maturation of Insulin-Secreting Cells In Vivo", STEM CELL, vol. 31, 2013, pages 2432 - 2442, XP055190599, DOI: 10.1002/stem.1489
ROBERT, THOMAS ET AL.: "Functional Beta Cell Mass from Device-Encapsulated hESC-Derived Pancreatic Endoderm Achieving Metabolic Control", STEM CELL REPORTS, vol. 10, 13 March 2018 (2018-03-13), pages 739 - 750, XP055693918
BRUIN, J. E. ET AL.: "Accelerating the in vivo maturation of macro-encapsulated human embryonic stem cell derived-pancreatic progenitor cells by manipulating NKX6. 1 expression in culture", DIABETOLOGIA, vol. 55, 2012, pages S178 - S179, XP009527010, ISSN: 0012-186X
REZANIA A. ET AL., NAT BIOTECHNOL., vol. 32, 2014, pages 1121 - 33
FELICIA W. PAGLIUCA ET AL., CELL, vol. 159, no. 2, 9 October 2014 (2014-10-09), pages 428 - 439
ROBERT T ET AL., STEM CELL REPORTS., vol. 10, no. 3, 13 March 2018 (2018-03-13), pages 739 - 750
TAKAHASHI KYAMANAKA S., CELL, vol. 126, 2006, pages 663 - 676
TAKAHASHI KYAMANAKA S. ET AL., CELL, vol. 131, 2007, pages 861 - 872
OKITA, K.ICHISAKA, T.YAMANAKA, S., NATURE, vol. 451, 2007, pages 141 - 146
HUANGFU D.MELTON, DA. ET AL., NATURE BIOTECHNOLOGY, vol. 26, no. 7, 2008, pages 795 - 797
OKITA K ET AL., NAT. METHODS, vol. 8, no. 5, May 2011 (2011-05-01), pages 409 - 12
OKITA K ET AL., STEM CELLS, vol. 31, no. 3, pages 458 - 66
YU J.THOMSON JA. ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
SHI Y.DING S. ET AL., CELL STEM CELL, vol. 3, no. 5, 2008, pages 568 - 574
KIM JB.SCHOLER HR. ET AL., NATURE, vol. 454, 2008, pages 646 - 650
TOYODA ET AL., STEM CELL RESEARCH, vol. 14, 2015, pages 185 - 197
NATURE BIOTECHNOLOGY, vol. 32, 2014, pages 1121 - 1133
See also references of EP 3854869A4
Attorney, Agent or Firm:
OHNO, Seiji et al. (JP)
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