Title:
LINOLEATE ISOMERASE AND USE THEREOF IN PRODUCTION OF CONJUGATE LINOLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2021/077543
Kind Code:
A1
Abstract:
Disclosed are a linoleate isomerase and the use thereof in the production of conjugate linoleic acid, which fall within the technical fields of protein engineering and microbial engineering. A linoleate isomerase derived from Bifidobacterium can be used for producing conjugate linoleic acid. By means of adding recombinant Escherichia coli containing linoleate isomerase into a reaction system containing linoleic acid for a 3 h reaction, it is possible for the conversion rate of conjugate linoleic acid to reach up to 12.1% to 42.1％ and the content of cis9, trans11-CLA in conjugate linoleic acid to reach up to 84.3％ to 89.1％, which provides theoretical support for further obtaining, by means of genetic engineering, microorganisms which are safe, have a high yield and in which most produced conjugate linoleic acid monomers are cis9,trans11-CLA.
Inventors:
CHEN HAIQIN (CN)
YANG BO (CN)
GAO HE (CN)
ZHAO JIANXIN (CN)
CHEN YONGQUAN (CN)
ZHANG HAO (CN)
CHEN WEI (CN)
YANG BO (CN)
GAO HE (CN)
ZHAO JIANXIN (CN)
CHEN YONGQUAN (CN)
ZHANG HAO (CN)
CHEN WEI (CN)
Application Number:
PCT/CN2019/121822
Publication Date:
April 29, 2021
Filing Date:
November 29, 2019
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/19; C12N9/90; C12N1/21
Foreign References:
| US9771624B2 | 2017-09-26 |
Other References:
DATABASE NUCLEOTIDE 4 December 2018 (2018-12-04), ANONYMOUS: "Bifidobacterium breve strain lw01 chromosome, complete genome", XP055805104, retrieved from GENBANK Database accession no. CP034192
DATABASE NUCLEOTIDE 30 April 2019 (2019-04-30), ANONYMOUS: "Bifidobacterium longum strain 51A chromosome, complete genome", XP055805154, retrieved from GENBANK Database accession no. CP026999
DATABASE NUCLEOTIDE 18 December 2018 (2018-12-18), ANONYMOUS: "Bifidobacterium pseudocatenulatum strain 12 chromosome, complete genome", XP055805163, retrieved from GENBANK Database accession no. CP025199
DATABASE NUCLOTIDE 19 December 2018 (2018-12-19), ANONYMOUS: "Bifidobacterium dentium strain NCTC11816 genome assembly, chromosome: 1", XP055805203, retrieved from GENBANK Database accession no. LR134349
ZHANG, YANHE ET AL.: "Development of linoleate isomerase catalyzes for production of conjugated linoleic acid", MICROBIOLOGY CHINA, vol. 38, no. 1, 20 January 2011 (2011-01-20), pages 105 - 109, XP055805207
DATABASE NUCLEOTIDE 30 April 2019 (2019-04-30), ANONYMOUS: "Bifidobacterium longum strain 51A chromosome, complete genome", XP055805154, retrieved from GENBANK Database accession no. CP026999
DATABASE NUCLEOTIDE 18 December 2018 (2018-12-18), ANONYMOUS: "Bifidobacterium pseudocatenulatum strain 12 chromosome, complete genome", XP055805163, retrieved from GENBANK Database accession no. CP025199
DATABASE NUCLOTIDE 19 December 2018 (2018-12-19), ANONYMOUS: "Bifidobacterium dentium strain NCTC11816 genome assembly, chromosome: 1", XP055805203, retrieved from GENBANK Database accession no. LR134349
ZHANG, YANHE ET AL.: "Development of linoleate isomerase catalyzes for production of conjugated linoleic acid", MICROBIOLOGY CHINA, vol. 38, no. 1, 20 January 2011 (2011-01-20), pages 105 - 109, XP055805207
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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