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Title:
MASS CULTURE OF PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2020/184350
Kind Code:
A1
Abstract:
This method for producing pluripotent stem cells comprises steps (a) and (b) below: (a) a step for filling a culture container with a liquid culture medium, and elevating the temperature of the liquid culture medium in the culture container to a temperature at which pluripotent cells can proliferate; and (b) a step for seeding the pluripotent stem cells to the liquid culture medium in the culture container, and performing a suspension cultivation.

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Inventors:
MATSUTA HIROTOSHI (JP)
UEDA YASUYOSHI (JP)
Application Number:
PCT/JP2020/009268
Publication Date:
September 17, 2020
Filing Date:
March 04, 2020
Export Citation:
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Assignee:
KANEKA CORP (JP)
International Classes:
C12N5/02; A61K35/12; C12N5/071; C12N5/074; C12N5/075; C12N5/076; C12N5/077; C12N5/078; C12N5/0783; C12N5/0784; C12N5/0789; C12N5/0793; C12N5/10
Domestic Patent References:
WO2016076368A12016-05-19
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WO2008117813A12008-10-02
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Foreign References:
JP2019042797A2019-03-22
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JP2013256510A2013-12-26
JP2016052272A2016-04-14
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Other References:
ANONYMOUS: "Q. The number of cells does not increase", 1 January 2017 (2017-01-01), JP, pages 1 - 1, XP009530536, Retrieved from the Internet
SHAMBLOTT M. J. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 95, 1998, pages 13726 - 13731
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Attorney, Agent or Firm:
OIKAWA Shu et al. (JP)
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