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Title:
MEDIUM FOR ENHANCING PROLIFERATION ABILITY OF NAIVE TYPE PLURIPOTENT STEM CELLS AND METHOD FOR PRODUCING PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2021/020267
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a medium that is capable of enhancing the proliferation ability of naive type pluripotent stem cells and a method for producing pluripotent stem cells having enhanced proliferation ability with the use of the medium. According to the present invention, provided is a medium for enhancing the proliferation ability of naive type pluripotent stem cells, said medium satisfying the following requirements (1) to (3). (1) Containing an MAPK/ERK kinase inhibitor and a GSK3β inhibitor. (2) Containing any member selected from the group consisting of a protein kinase C inhibitor, a Wnt signal inhibitor and a STAT3 activator. (3) Containing an NFAT inhibitor. According to the present invention, also provided is a method for producing pluripotent stem cells, said method comprising a step for culturing naive type pluripotent stem cells in the aforesaid medium.

Inventors:
YAMAZAKI NAO (JP)
MURAGUCHI TAICHI (JP)
WATANABE SHINICHI (JP)
Application Number:
PCT/JP2020/028403
Publication Date:
February 04, 2021
Filing Date:
July 22, 2020
Export Citation:
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Assignee:
FUJIFILM CORP (JP)
International Classes:
C12N5/0735; C12N1/00; C12N5/074
Domestic Patent References:
WO2015147047A12015-10-01
Foreign References:
JP2016521971A2016-07-28
US20180112187A12018-04-26
CN102382796A2012-03-21
Other References:
GUO, G. ET AL.: "Epigenetic resetting of human pluripotency", DEVELOPMENT, vol. 144, 2017, pages 2748 - 2763, XP055456607, DOI: 10.1242/dev.146811
LI XIANG, ZHU LILI, YANG ACONG, LIN JIANGWEI, TANG FAN, JIN SHIBO, WEI ZHE, LI JINSONG, JIN YING: "Calcineurin-NFAT signaling critically regulates early lineage specification in mouse embryonic stem cells and embryos", CELL STEM CELL, vol. 8, no. 1, 7 January 2011 (2011-01-07), pages 46 - 58, XP055788092
Attorney, Agent or Firm:
SIKS & CO. (JP)
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