Title:
METHOD FOR BASE SEQUENCING AND BIOLOGICALLY ACTIVE NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO2002044195
Kind Code:
A3
Abstract:
Aptamers are nucleic acids and similar molecules, such as peptide-nucleic acids, that specifically bind to a ligand such as a protein or peptide. The present invention provides aptamers comprising at least one base capable of base pairing and different from the standard Watson-Crick bases. The present invention also relates to a method for preparation of such aptamers and to methods for sequencing nucleic acids that comprise at least one base capable of base pairing and different from the standard Watson-Crick bases.
Inventors:
HAYASHIZAKI YOSHIHIDE (JP)
Application Number:
PCT/JP2001/010400
Publication Date:
March 27, 2003
Filing Date:
November 28, 2001
Export Citation:
Assignee:
RIKEN (JP)
DNAFORM KK (JP)
HAYASHIZAKI YOSHIHIDE (JP)
DNAFORM KK (JP)
HAYASHIZAKI YOSHIHIDE (JP)
International Classes:
C07F9/09; C07H19/10; C07H19/20; C07H21/00; C07H21/04; C12N15/115; C12Q1/68; C12Q1/6869; C40B30/04; G01N33/53; G01N33/68; G01N37/00; (IPC1-7): C12Q1/68; C07H21/00; C12N15/10; C12N15/11; C12P19/34; G01N33/53; C07H19/20; C07H19/10; C07B61/00
Foreign References:
| US6001983A | 1999-12-14 | |||
| US5432272A | 1995-07-11 |
Other References:
ROBERTS C ET AL: "Synthesis of Oligonucleotides Bearing the Non-Standard Bases iso-C and iso-G. Comparison of iso-C-iso-G, C-G and U-A Base-Pair Stabilities in RNA/DNA Duplexes", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 36, no. 21, 22 May 1995 (1995-05-22), pages 3601 - 3604, XP004028029, ISSN: 0040-4039
HERMANN T. AND PATEL D. J.: "Adaptive recognition by nucleic acid aptamers", SCIENCE, vol. 287, 4 February 2000 (2000-02-04), pages 820 - 825, XP002208494
COLLINS M.L. ET AL: "A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml", NUCLEIC ACIDS RESEARCH, vol. 25, no. 15, 1997, pages 2979 - 2984, XP002208495
TUERK C ET AL: "SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT: RNA LIGANDS TO BACTERIOPHAGE T4 DNA POLYMERASE", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 249, 3 August 1990 (1990-08-03), pages 505 - 510, XP000647748, ISSN: 0036-8075
HERMANN T. AND PATEL D. J.: "Adaptive recognition by nucleic acid aptamers", SCIENCE, vol. 287, 4 February 2000 (2000-02-04), pages 820 - 825, XP002208494
COLLINS M.L. ET AL: "A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml", NUCLEIC ACIDS RESEARCH, vol. 25, no. 15, 1997, pages 2979 - 2984, XP002208495
TUERK C ET AL: "SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT: RNA LIGANDS TO BACTERIOPHAGE T4 DNA POLYMERASE", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 249, 3 August 1990 (1990-08-03), pages 505 - 510, XP000647748, ISSN: 0036-8075
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