Title:
METHOD, COMPOSITION AND KIT FOR SIZE SELECTIVE ENRICHMENT OF NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2021/037075
Kind Code:
A1
Abstract:
Provided is a method for isolating and concentrating nucleic acids of selected target sizes (e.g., in increments less than 1000 base pairs) from a biological fluid mixture comprising combining the biological fluid mixture and a first aqueous two-phase system (ATPS) formed from a first phase forming polymer or surfactant component dissolved in a first phase solution, and a second phase solution, such that target nucleic acid fragments below a desired target size partition to said second phase solution and contaminants partition to the first phase solution, extracting and mixing the second phase solution with a second ATPS formed from a second phase forming polymer or surfactant component dissolved in a third phase solution and a fourth phase solution, such that the target nucleic acid fragments partition to and concentrate in the third phase solution, and recovering the concentrated target nucleic acid fragments from the third phase solution. A composition and kit for isolating and concentrating nucleic acids of selected target sizes as described above are also provided.
Inventors:
CHIU YIN TO (CN)
MARSHAK DANIEL (US)
KITTUR HARSHA MADAN (US)
KOBAYASHI MASAE (US)
MARSHAK DANIEL (US)
KITTUR HARSHA MADAN (US)
KOBAYASHI MASAE (US)
Application Number:
PCT/CN2020/111449
Publication Date:
March 04, 2021
Filing Date:
August 26, 2020
Export Citation:
Assignee:
PHASE SCIENT INTERNATIONAL LTD (CN)
International Classes:
C12Q1/68; C07H21/04
Domestic Patent References:
| WO2019055926A2 | 2019-03-21 | |||
| WO2019144030A1 | 2019-07-25 | |||
| WO2018183454A1 | 2018-10-04 |
Foreign References:
| CN106662582A | 2017-05-10 | |||
| CN110003323A | 2019-07-12 |
Other References:
IQBAL，M. ET AL.: "Aqueous two-phase system (ATPS): an overview and advances in its applications", BIOLOGICAL PROCEDURES ONLINE, vol. 18, 28 October 2016 (2016-10-28), pages 1 - 18, XP021264692
HIRASAKI ET AL., J. MEMBR. SCI., vol. 106, 1995, pages 123 - 129
FONG ET AL., CLINICAL CHEMISTRY, vol. 55, no. 3, 2009, pages 587 - 589
MATOS ET AL., SEP. PURIF. TECHNOL., vol. 122, 2014, pages 144 - 148
RIBEIRO, BIOTECHNOL. BIOENG., vol. 78, no. 4, 20 May 2002 (2002-05-20), pages 376 - 84
See also references of EP 4022081A4
HIRASAKI ET AL., J. MEMBR. SCI., vol. 106, 1995, pages 123 - 129
FONG ET AL., CLINICAL CHEMISTRY, vol. 55, no. 3, 2009, pages 587 - 589
MATOS ET AL., SEP. PURIF. TECHNOL., vol. 122, 2014, pages 144 - 148
RIBEIRO, BIOTECHNOL. BIOENG., vol. 78, no. 4, 20 May 2002 (2002-05-20), pages 376 - 84
See also references of EP 4022081A4
Attorney, Agent or Firm:
EAGLE IP (SHEN ZHEN) LIMITED (CN)
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