Title:
METHOD FOR CULTURING CELL POPULATION AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/196615
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a means for efficiently preparing a cell population, which contains abundant cells having a definite characteristic corresponding to the purpose of use (for example, type II collagen-positive nucleus pulposus cells), from a cell population containing Tie2-positive stem/progenitor cells (for example, nucleus pulposus stem/progenitor cells). The culture method according to the present invention comprises culturing a cell population containing Tie2-positive stem/progenitor cells while suppressing the formation of spherical colonies under the following conditions: (1) in a state of being present in a tissue which is not subjected to a digestion treatment; (2) in a medium which contains at least one Tie2 expression enhancer other than a growth factor; (3) in a culture container which is provided with a culture surface having been treated so as to enhance cell adhesion; or (4) in a medium which contains an extracellular matrix degrading agent.
Inventors:
SAKAI DAISUKE (JP)
NAKAMURA YOSHIHIKO (JP)
MATSUSHITA ERIKA (JP)
NAKAMURA YOSHIHIKO (JP)
MATSUSHITA ERIKA (JP)
Application Number:
PCT/JP2020/013307
Publication Date:
October 01, 2020
Filing Date:
March 25, 2020
Export Citation:
Assignee:
TOKAI UNIV EDUCATIONAL SYSTEM (JP)
NIPPON ZOKI PHARMACEUTICAL CO (JP)
NIPPON ZOKI PHARMACEUTICAL CO (JP)
International Classes:
C12N5/077
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Other References:
HARO H. ET AL.: "Experimental studies on the effects of recombinant human matrix metalloproteinases on herniated disc tissues - how to facilitate the natural resorption process of herniated discs.", JOURNAL OF ORTHOPAEDIC RESEARCH, vol. 23, 2005, pages 412 - 419, XP004763669, DOI: 10.1016/j.orthres.2004.08.020
TANAKA, MASAHIRO: "O-13-7: A basic study on the cryopreservation method of activated nucleus pulposus cells", REGENERATIVE MEDICINE, vol. 9, no. Suppl. 2010, 30 November 2009 (2009-11-30) - 6 March 2009 (2009-03-06), JP , pages 182, XP009523720, ISSN: 1347-7919
HARO, H. ET AL: "Experimental studies on the effects of recombinant human matrix metalloproteinases on herniated disc tissues", JOURNAL OF ORTHOPAEDIC RESEARCH, pages 412 - 419
SAKAI D ET AL., NAT COMMUN, vol. 3, 2012, pages 1264
TANAKA, MASAHIRO: "O-13-7: A basic study on the cryopreservation method of activated nucleus pulposus cells", REGENERATIVE MEDICINE, vol. 9, no. Suppl. 2010, 30 November 2009 (2009-11-30) - 6 March 2009 (2009-03-06), JP , pages 182, XP009523720, ISSN: 1347-7919
HARO, H. ET AL: "Experimental studies on the effects of recombinant human matrix metalloproteinases on herniated disc tissues", JOURNAL OF ORTHOPAEDIC RESEARCH, pages 412 - 419
SAKAI D ET AL., NAT COMMUN, vol. 3, 2012, pages 1264
Attorney, Agent or Firm:
UMEDA Shinsuke et al. (JP)
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