Title:
METHOD OF DETECTING INFECTION WITH PATHOGENS CAUSING TUBERCULOSIS
Document Type and Number:
WIPO Patent Application WO/2020/127908
Kind Code:
A3
Abstract:
The present invention refers to in vitro methods of detecting an infection with pathogens causing tuberculosis comprising the steps of (a) contacting a first aliquot of a sample of an individual with at least one antigen of a pathogen causing tuberculosis, b) incubating the first aliquot with the at least one antigen over a certain period of time, c) detecting in the first aliquot and in a second aliquot of the sample of the individual a marker or a combination of markers, e.g. Interferon gamma, CXCL10, ncTRIM69, using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) or RNA Sequencing (RNA-Seq), and d) comparing the detected marker(s) in the first aliquot with the detected marker(s) in the second aliquot, wherein the second aliquot has not been incubated with the at least one antigen. In addition, the present invention refers to a kit for performing the methods according to the present invention. The present invention also refers to the use of the marker ncTRIM69, a primer for amplification of the marker ncTRIM69, and/or a probe for detecting the marker ncTRIM69 in an / n vitro method of diagnosing tuberculosis, in particular of detecting infection with pathogens causing tuberculosis.
Inventors:
DEML LUDWIG (DE)
RASCLE ANNE (DE)
BARABAS SASCHA (DE)
ASBACH-NITZSCHE ALEXANDRA (DE)
MEIER JOHANNES P (DE)
RASCLE ANNE (DE)
BARABAS SASCHA (DE)
ASBACH-NITZSCHE ALEXANDRA (DE)
MEIER JOHANNES P (DE)
Application Number:
PCT/EP2019/086579
Publication Date:
July 30, 2020
Filing Date:
December 20, 2019
Export Citation:
Assignee:
LOPHIUS BIOSCIENCES GMBH (DE)
International Classes:
C12Q1/6883
Foreign References:
| US20130288229A1 | 2013-10-31 | |||
| US20120015386A1 | 2012-01-19 | |||
| KR20110116644A | 2011-10-26 |
Other References:
KUMAR NATHELLA PAVAN ET AL: "Modulation of Th1/Tc1 and Th17/Tc17 responses in pulmonary tuberculosis by IL-20 subfamily of cytokines", CYTOKINE, ACADEMIC PRESS LTD, PHILADELPHIA, PA, US, vol. 108, 21 April 2018 (2018-04-21), pages 190 - 196, XP085399176, ISSN: 1043-4666, DOI: 10.1016/J.CYTO.2018.04.005
W RAY WATERS ET AL: "Interleukin-17A as a Biomarker for Bovine Tuberculosis", CLINICAL AND VACCINE IMMUNOLOGY : CVI, 1 February 2016 (2016-02-01), United States, pages 168, XP055514481, Retrieved from the Internet DOI: 10.1128/CVI.00637-15
SUNGHYUN KIM ET AL: "Diagnostic Performance of a Cytokine and IFN-[gamma]-Induced Chemokine mRNA Assay after Mycobacterium tuberculosis-Specific Antigen Stimulation in Whole Blood from Infected Individuals", THE JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 17, no. 1, 1 January 2015 (2015-01-01), US, pages 90 - 99, XP055514324, ISSN: 1525-1578, DOI: 10.1016/j.jmoldx.2014.08.005
W RAY WATERS ET AL: "Interleukin-17A as a Biomarker for Bovine Tuberculosis", CLINICAL AND VACCINE IMMUNOLOGY : CVI, 1 February 2016 (2016-02-01), United States, pages 168, XP055514481, Retrieved from the Internet
SUNGHYUN KIM ET AL: "Diagnostic Performance of a Cytokine and IFN-[gamma]-Induced Chemokine mRNA Assay after Mycobacterium tuberculosis-Specific Antigen Stimulation in Whole Blood from Infected Individuals", THE JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 17, no. 1, 1 January 2015 (2015-01-01), US, pages 90 - 99, XP055514324, ISSN: 1525-1578, DOI: 10.1016/j.jmoldx.2014.08.005
Attorney, Agent or Firm:
DREXL, JANNA (DE)
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