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Title:
METHOD FOR DETECTING ORGANIC ACID IN SAMPLE
Document Type and Number:
WIPO Patent Application WO/2014/126207
Kind Code:
A1
Abstract:
Provided is a method whereby an organic acid, in particular an organic acid such as 3-HB, 3-HIB, etc. potentially usable as a biomarker, in a sample can be highly sensitively and highly accurately detected. The method for detecting an organic acid in a sample is characterized by comprising: ester-binding the organic acid in the sample to a derivatizing reagent represented by any of general formulae (1)-1 to (1)-3 [wherein one of R1 and R2 represents a C1-6 hydroxyalkyl group and the other represents a hydrogen atom or a C1-6 alkyl group] to synthesize a derivative; and detecting the derivative by the positive ion mode electrospray ionization LC-MS/MS (liquid chromatography-tandem mass spectrometry) method.

Inventors:
MATSUZAKI YASUSHI (JP)
HONDA AKIRA (JP)
MIYAZAKI TERUO (JP)
IKEGAMI TADASHI (JP)
Application Number:
PCT/JP2014/053506
Publication Date:
August 21, 2014
Filing Date:
February 14, 2014
Export Citation:
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Assignee:
AJINOMOTO KK (JP)
UNIV TOKYO MEDICAL (JP)
International Classes:
G01N27/62; G01N30/06; G01N30/72; G01N30/88; G01N33/487
Domestic Patent References:
WO2012049874A12012-04-19
Foreign References:
JP2007132741A2007-05-31
JP2011247869A2011-12-08
Other References:
JUTILA,M. ET AL.: "Piperidyl and morpholinyl esters as derivatives for the structural determination of long-chain fatty acids by mass spectrometry", BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, vol. 17, December 1988 (1988-12-01), pages 433 - 436
HONDA,A. ET AL.: "Highly sensitive quantification of serum malonate, a possible marker for de novo lipogenesis, by LC-ESI-MS/MS", JOURNAL OF LIPID RESEARCH, vol. 50, October 2009 (2009-10-01), pages 2124 - 2130
TERUO MIYAZAKI: "Kokkakukin Branched Chain Amino Acid Ika Marker to shite no Kecchu 3-Hydroxyisobutyric Acid Sokutei no Yuyosei", JAPANESE JOURNAL OF PHYSICAL FITNESS AND SPORTS MEDICINE, vol. 61, no. 6, 1 December 2012 (2012-12-01), pages 632
TERUO MIYAZAKI: "Shokaki Shikkan Kanja ni Okeru Kecchu 3-Hydroxyisobutyric Acid Nodo no Hikaku", ACTA HEPATOLOGICA JAPONICA, vol. 53, no. SUPPL, 10 September 2012 (2012-09-10), pages A649
DESHPANDE,P.: "Development and validation of LC-MS/MS method for the estimation of beta-hydroxy-beta-methylbutyrate in rat plasma and its application to pharmacokinetic studies", BIOMEDICAL CHROMATOGRAPHY, vol. 27, 24 May 2012 (2012-05-24), pages 142 - 147
TERUO MIYAZAKI: "Kankohen Kanja no Kakkakukin ni Okeru Shibosan beta Sanka Kassei no Hyoka", ACTA HEPATOLOGICA JAPONICA, vol. 54, no. SUPPLE, 25 April 2013 (2013-04-25), pages A175
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
Masatake Shiga (JP)
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