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Title:
METHOD FOR DETECTING TARGET NUCLEIC ACID USING DRIED BLOOD FILTER PAPER PIECE
Document Type and Number:
WIPO Patent Application WO/2021/167058
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method that is for detecting target nucleic acid in dried blood filter paper by real-time PCR using a fluorescent dye, and in which an influence of a disturbance of a base line is reduced by a simple method. The present invention provides a method that is for detecting target nucleic acid in dried blood filter paper by real-time PCR, and that comprises: (1) a step for subjecting a sample solution that contains a punched piece of dried blood filter paper and a PCR reagent containing a fluorescently-labeled probe, to thermal cycles to amplify the target nucleic acid in the dried blood filter paper; (2) a step for optically detecting the fluorescence intensity of the sample solution in each of the thermal cycles; and (3) a step for performing quantitative analysis of the target nucleic acid using data obtained after a predetermined number of cycles of the optically detected data.

Inventors:
EBINUMA HIROYUKI (JP)
SUZUKI IKUMI (JP)
Application Number:
PCT/JP2021/006319
Publication Date:
August 26, 2021
Filing Date:
February 19, 2021
Export Citation:
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Assignee:
SEKISUI MEDICAL CO LTD (JP)
International Classes:
C12N15/12; C12N9/00; C12Q1/686; C12Q1/6876
Domestic Patent References:
WO2018181850A12018-10-04
WO2018025856A12018-02-08
WO2019074004A12019-04-18
Foreign References:
JP2019166510A2019-10-03
JP2004283165A2004-10-14
JP5144518B22013-02-13
JP2008545380W
Attorney, Agent or Firm:
MOEGI PATENT OFFICE (JP)
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