Title:
METHOD FOR DETECTING TARGET NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2021/199770
Kind Code:
A1
Abstract:
The present invention pertains to a method for detecting a target nucleic acid, the method including: cleaving a first flap of a first cleavage structure formed by a target nucleic acid, a first nucleic acid, and a second nucleic acid; cleaving a second flap of a second cleavage structure formed by a third nucleic acid, the cleaved first flap, and a fourth nucleic acid; and detecting the presence of the target nucleic acid by detecting the cleaved second flap, cleavage of the first flap and cleavage of the second flap being carried out by cleaving the first flap and the second flap using a flap endonuclease, and the flap endonuclease having an amino acid sequence that has 96% or greater sequence identity with an amino acid sequence of a flap endonuclease of Thermococcus kodakarensis KOD1, etc.
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Inventors:
MAKINO YOICHI (JP)
ISHINO YOSHIZUMI (JP)
ISHINO SONOKO (JP)
ISHINO YOSHIZUMI (JP)
ISHINO SONOKO (JP)
Application Number:
PCT/JP2021/006198
Publication Date:
October 07, 2021
Filing Date:
February 18, 2021
Export Citation:
Assignee:
TOPPAN INC (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N15/55; C12N15/63; C12Q1/34; C12Q1/6813
Domestic Patent References:
| WO2019026884A1 | 2019-02-07 |
Foreign References:
| JP6846763B1 | 2021-03-24 | |||
| JP2001518805A | 2001-10-16 |
Other References:
DATABASE PROTEIN 29 September 2018 (2018-09-29), ANONYMOUS : "flap structure-specific endonuclease [Thermococcus kodakarensis KOD1] ", XP055935618, retrieved from NCBI Database accession no. BAD85470.1
DATABASE Protein 7 March 2015 (2015-03-07), ANONYMOUS : "fen1 FLAP endonuclease-1 [Pyrococcus abyssi GE5]", XP055935619, retrieved from NCBI Database accession no. CAB49654.1
DATABASE UniProt [online] 2003, "Flap endonuclease 1", Database accession no. 027670
YOICHI MAKINO, HIROSHI USHIDA: "Development of digital biomolecule detection system", JOURNAL OF PRINTING SCIENCE AND TECNOLOGY, vol. 54, no. 6, 1 January 2017 (2017-01-01), pages 377 - 382, XP055952714, ISSN: 0914-3319, DOI: 10.11413/nig.54.377
DATABASE PROTEIN 29 March 2021 (2021-03-29), ANONYMOUS : "flap endonuclease-1 [Thermococcus gorgonarius]", XP055935622, retrieved from NCBI Database accession no. WP_088884722.1
DATABASE PROTEIN 29 March 2021 (2021-03-29), ANONYMOUS : "flap endonuclease-1 [Pyrococcus horikoshii] ", XP055935628, retrieved from NCBI Database accession no. WP010885498.1
JOURNAL OF PRINTING SCIENCE AND TECHNOLOGY, vol. 54, no. 6, 2017, pages 377 - 382
See also references of EP 4092122A4
DATABASE Protein 7 March 2015 (2015-03-07), ANONYMOUS : "fen1 FLAP endonuclease-1 [Pyrococcus abyssi GE5]", XP055935619, retrieved from NCBI Database accession no. CAB49654.1
DATABASE UniProt [online] 2003, "Flap endonuclease 1", Database accession no. 027670
YOICHI MAKINO, HIROSHI USHIDA: "Development of digital biomolecule detection system", JOURNAL OF PRINTING SCIENCE AND TECNOLOGY, vol. 54, no. 6, 1 January 2017 (2017-01-01), pages 377 - 382, XP055952714, ISSN: 0914-3319, DOI: 10.11413/nig.54.377
DATABASE PROTEIN 29 March 2021 (2021-03-29), ANONYMOUS : "flap endonuclease-1 [Thermococcus gorgonarius]", XP055935622, retrieved from NCBI Database accession no. WP_088884722.1
DATABASE PROTEIN 29 March 2021 (2021-03-29), ANONYMOUS : "flap endonuclease-1 [Pyrococcus horikoshii] ", XP055935628, retrieved from NCBI Database accession no. WP010885498.1
JOURNAL OF PRINTING SCIENCE AND TECHNOLOGY, vol. 54, no. 6, 2017, pages 377 - 382
See also references of EP 4092122A4
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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