Title:
METHOD FOR DETERMINING SINGLE BASE MUTATION OF ERM (41) GENE OF ACID-FAST BACILLUS BELONGING TO MYCOBACTEROIDES ABSCESSUS COMPLEX, AND PRIMER SET AND PROBE USED IN SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2021/201206
Kind Code:
A1
Abstract:
One or more embodiments of the present invention provides a novel means for determining a single base mutation of erm (41) gene of acid-fast bacillus belonging to Mycobacteroides abscessus complex. One or more embodiments relate to a method for determining a base corresponding to position 28 of erm (41) gene of acid-fast bacillus belonging to Mycobacteroides abscessus complex, the method including detection of an index base sequence in the genome DNA of acid-fast bacillus which is a subject for determination, where the index base sequence is present in a region of the genome DNA other than the erm (41) gene when the base of the erm (41) gene is cytosine, and is not present when the base is thymine, thereby establishing that the presence of the index base sequence indicates that the base is cytosine, and the absence of the index base sequence indicates that the base is thymine.
Inventors:
HOSHINO YOSHIHIKO (JP)
YOSHIDA MITSUNORI (JP)
SANO SOTARO (JP)
MIYAMOTO SHIGEHIKO (JP)
YOSHIDA MITSUNORI (JP)
SANO SOTARO (JP)
MIYAMOTO SHIGEHIKO (JP)
Application Number:
PCT/JP2021/014128
Publication Date:
October 07, 2021
Filing Date:
April 01, 2021
Export Citation:
Assignee:
JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NAT INSTITUTE OF INFECTIOUS DISEASES (JP)
KANEKA CORP (JP)
KANEKA CORP (JP)
International Classes:
C12N15/11; C12Q1/689
Domestic Patent References:
| WO2013136818A1 | 2013-09-19 | |||
| WO2012070618A1 | 2012-05-31 |
Foreign References:
| JP3859678B2 | 2006-12-20 | |||
| JP2020066277A | 2020-04-30 |
Other References:
MASE AYAKA, YAMAGUCHI FUMIHIRO, FUNAKI TOSHITAKA, YAMAZAKI YOHEI, SHIKAMA YUSUKE, FUKUCHI KUNIHIKO: "PCR amplification of the erm(41) gene can be used to predict the sensitivity of Mycobacterium�abscessus complex strains to clarithromycin", EXPERIMENTAL AND THERAPEUTIC MEDICINE, SPANDIDOS PUBLICATIONS, GR, 5 December 2019 (2019-12-05), GR , XP055923829, ISSN: 1792-0981, DOI: 10.3892/etm.2019.8289
LIPWORTH SAMUEL, HOUGH NATASHA, LEACH LAURA, MORGAN MARCUS, JEFFERY KATIE, ANDERSSON MONIQUE, ROBINSON ESTHER, SMITH E GRACE, CROO: "Whole-Genome Sequencing for Predicting Clarithromycin Resistance in Mycobacterium abscessus", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1 January 2019 (2019-01-01), XP055923862, [retrieved on 20220523], DOI: 10.1128/AAC.01204-18
WUZINSKI MICHELLE, BAK ANETAK, PETKAU AARON, B. DEMCZUK WALTERH, SOUALHINE HAFID, SHARMA MEENUKAUSHAL: "A multilocus sequence typing scheme for Mycobacterium abscessus complex (MAB-multilocus sequence typing) using whole-genome sequencing data", INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY, ELSEVIER, vol. 8, no. 3, 1 January 2019 (2019-01-01), pages 273, XP055880392, ISSN: 2212-5531, DOI: 10.4103/ijmy.ijmy_106_19
YOSHIDA MITSUNORI, SANO SOTARO, CHIEN JUNG-YIEN, FUKANO HANAKO, SUZUKI MASATO, ASAKURA TAKANORI, MORIMOTO KOZO, MURASE YOSHIRO, MI: "A novel DNA chromatography method to discriminate Mycobacterium abscessus subspecies and macrolide susceptibility", EBIOMEDICINE, ELSEVIER BV, NL, vol. 64, 11 January 2021 (2021-01-11), NL , XP055923851, ISSN: 2352-3964, DOI: 10.1016/j.ebiom.2020.103187
J ANTIMICROB CHEMOTHER, vol. 72, 2017, pages 1669 - 1677
GREENSAMBROOK: "Molecular Cloning", 2012, COLD SPRING HARBOR LABORATORY PRESS
ALTSCHU ET AL., NUC. ACIDS. RES, vol. 25, 1977, pages 3389 - 3402
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
LIPWORTH SAMUEL, HOUGH NATASHA, LEACH LAURA, MORGAN MARCUS, JEFFERY KATIE, ANDERSSON MONIQUE, ROBINSON ESTHER, SMITH E GRACE, CROO: "Whole-Genome Sequencing for Predicting Clarithromycin Resistance in Mycobacterium abscessus", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1 January 2019 (2019-01-01), XP055923862, [retrieved on 20220523], DOI: 10.1128/AAC.01204-18
WUZINSKI MICHELLE, BAK ANETAK, PETKAU AARON, B. DEMCZUK WALTERH, SOUALHINE HAFID, SHARMA MEENUKAUSHAL: "A multilocus sequence typing scheme for Mycobacterium abscessus complex (MAB-multilocus sequence typing) using whole-genome sequencing data", INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY, ELSEVIER, vol. 8, no. 3, 1 January 2019 (2019-01-01), pages 273, XP055880392, ISSN: 2212-5531, DOI: 10.4103/ijmy.ijmy_106_19
YOSHIDA MITSUNORI, SANO SOTARO, CHIEN JUNG-YIEN, FUKANO HANAKO, SUZUKI MASATO, ASAKURA TAKANORI, MORIMOTO KOZO, MURASE YOSHIRO, MI: "A novel DNA chromatography method to discriminate Mycobacterium abscessus subspecies and macrolide susceptibility", EBIOMEDICINE, ELSEVIER BV, NL, vol. 64, 11 January 2021 (2021-01-11), NL , XP055923851, ISSN: 2352-3964, DOI: 10.1016/j.ebiom.2020.103187
J ANTIMICROB CHEMOTHER, vol. 72, 2017, pages 1669 - 1677
GREENSAMBROOK: "Molecular Cloning", 2012, COLD SPRING HARBOR LABORATORY PRESS
ALTSCHU ET AL., NUC. ACIDS. RES, vol. 25, 1977, pages 3389 - 3402
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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