Title:
METHOD OF DISPLAYING PROTEIN
Document Type and Number:
WIPO Patent Application WO/2006/123445
Kind Code:
A1
Abstract:
A method of displaying a protein comprising the steps of translating an mRNA in a sample using a cell extract solution, and, by skipping at least one kind of stop codon at this time, obtaining a complex containing a combined body of mRNA and a protein produced by the translation thereof is provided. When the mRNA in the sample is translated using the cell extract solution, at least one kind of stop codon can be skipped by adding at least one kind of mutated tRNA in which the anticodon of amino acid tRNA has been replaced with an anticodon corresponding to the stop codon.
Inventors:
AOYAMA YASUHIRO (JP)
SANDO SHINSUKE (JP)
OGAWA ATSUSHI (JP)
SANDO SHINSUKE (JP)
OGAWA ATSUSHI (JP)
Application Number:
PCT/JP2005/018680
Publication Date:
November 23, 2006
Filing Date:
October 11, 2005
Export Citation:
Assignee:
UNIV KYOTO (JP)
AOYAMA YASUHIRO (JP)
SANDO SHINSUKE (JP)
OGAWA ATSUSHI (JP)
AOYAMA YASUHIRO (JP)
SANDO SHINSUKE (JP)
OGAWA ATSUSHI (JP)
International Classes:
C12N15/00; C12P21/00
Domestic Patent References:
| WO2005007870A2 | 2005-01-27 |
Foreign References:
| JPH05503000A | 1993-05-27 |
Other References:
HANES J AND PLUCKTHUN A.: "In vitro selection and evolution of functional proteins by using ribosome display.", PROC NATL ACAD SCI U S A., vol. 94, 1997, pages 4937 - 4942, XP002079230
AGAFONIV D E ET AL: "Efficient suppression of the amber codon in E. coli in vitro translation system.", FEBS LETT., vol. 579, April 2005 (2005-04-01), pages 2156 - 2160, XP004825007
LI S ET AL: "In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid.", J AM CHEM SOC., vol. 124, 2002, pages 9972 - 9973, XP002970893
AGAFONIV D E ET AL: "Efficient suppression of the amber codon in E. coli in vitro translation system.", FEBS LETT., vol. 579, April 2005 (2005-04-01), pages 2156 - 2160, XP004825007
LI S ET AL: "In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid.", J AM CHEM SOC., vol. 124, 2002, pages 9972 - 9973, XP002970893
Attorney, Agent or Firm:
Saegusa, Eiji (1-7-1 Doshomachi, Chuo-k, Osaka-shi Osaka, JP)
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