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Title:
METHOD FOR EFFICIENT CATALYTIC SYNTHESIS OF PAPS BASED ON CONSTRUCTION OF ATP REGENERATION SYSTEM
Document Type and Number:
WIPO Patent Application WO/2022/068089
Kind Code:
A1
Abstract:
Provided is a method for catalytic synthesis of PAPS based on the construction of an ATP regeneration system, comprising artificially constructing a bifunctional PAPS synthesis enzyme by means of microbial recombinant expression. On the basis of the ATP regeneration system coupling the polyphosphate kinase derived from Corynebacterium glutamicum and Mycobacterium tuberculosis, two by-products, pyrophosphoric acid and ADP, can be recovered at the same time, the equivalent conversion of substrate and product can be achieved, the PAPS generated in the catalytic system has high purity, and the supply of sulfonic acid groups in most of sulfonic acid transfer reactions can be achieved.

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Inventors:
KANG ZHEN (CN)
DU GUOCHENG (CN)
WANG YANG (CN)
XU RUIRUI (CN)
CHEN JIAN (CN)
Application Number:
PCT/CN2020/137172
Publication Date:
April 07, 2022
Filing Date:
December 17, 2020
Export Citation:
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Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12P19/32; C12N1/21; C12N9/12; C12N15/09; C12N15/54; C12N15/55; C12R1/15; C12R1/32; C12R1/465
Foreign References:
CN101107356A2008-01-16
CN110982864A2020-04-10
Other References:
WANG YANXING: "Studies on the Mechanism of Substrate Channeling in Sulfate Activating Complex of Rhodobacter Sphaeroides", BASIC SCIENCES, CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 5, 15 May 2012 (2012-05-15), XP055918232
HUANG YUANBO: "Construction of Sulfate Activating Complex and Pyrophosphatase Insertion Mutants of Rhodobacter Sphaeroides", BASIC SCIENCES, CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 3, 15 March 2014 (2014-03-15), XP055918230
LI HONGYAN; WANG YANXING; MA JIANHUI; SUN MEIHAO: "Analysis of the Structural Basis for the Chanelling in Sulfate-Activating Complexes", PROCEEDINGS OF THE TENTH SYMPOSIUM OF THE CHINESE ENZYME SOCIETY; 2011-10-22, CHINESE SOCIETY OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, CN, 22 October 2011 (2011-10-22), CN, pages 37, XP009535968
XIE LIPING, HU YOU-JIA, ZHU CHUN-BAO: "Application of Cofactor Regeneration in Biotransformation", PHARMACEUTICAL BIOTECHNOLOGY, vol. 14, no. 6, 15 December 2007 (2007-12-15), pages 460 - 464, XP055918226, ISSN: 1005-8915, DOI: 10.19526/j.cnki.1005-8915.2007.06.017
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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