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Patent Searching and Data


Title:
METHOD FOR ENZYMATICALLY PRODUCING MAYTANSINOL
Document Type and Number:
WIPO Patent Application WO/2021/107133
Kind Code:
A8
Abstract:
Provided is a method for producing maytansinol, the method including a step for enzymatically generating maytansinol from ansamitocins using any protein among (A)-(F) and (AA)-(CC): (A) a protein comprising the amino acid sequence represented by SEQ ID NO: 9; (B) a protein comprising the amino acid sequence represented by SEQ ID NO: 9 with one or more amino acids substituted, deleted, inserted and/or added, and having an activity that catalyzes a reaction that produces maytansinol from ansamitocins; (C) a protein having at least 50% amino acid identity with the amino acid sequence represented by SEQ ID NO: 9, and having an activity that catalyzes a reaction that produces maytansinol from ansamitocins; (D) a protein comprising the amino acid sequence represented by SEQ ID NO: 12; (E) a protein comprising the amino acid sequence represented by SEQ ID NO: 12 with one or more amino acids substituted, deleted, inserted and/or added, and having an activity that catalyzes a reaction that produces maytansinol from ansamitocins; (F) a protein having at least 50% amino acid identity with the amino acid sequence represented by SEQ ID NO: 12, and having an activity that catalyzes a reaction that produces maytansinol from ansamitocins; (AA) a protein comprising the amino acid sequence represented by any one of SEQ ID NOs:18-21; (BB) a protein comprising the amino acid sequence represented by any one of SEQ ID NOs:18-21 with one or more amino acids substituted, deleted, inserted and/or added, and having an activity that catalyzes a reaction that produces maytansinol from ansamitocins; and (CC) a protein having at least 80% amino acid identity with the amino acid sequence represented by any one of SEQ ID NOs:18-21, and having an activity that catalyzes a reaction that produces maytansinol from ansamitocins.

Inventors:
BABA YUTARO (JP)
NISHIMURA KENJI (JP)
MIKI YUTA (JP)
KAWASAKI YUKIE (JP)
YAMAMOTO TSUYOSHI (JP)
KAWASAKI MAYU (JP)
NAKANO SHOGO (JP)
ITO SOHEI (JP)
Application Number:
PCT/JP2020/044347
Publication Date:
September 30, 2021
Filing Date:
November 27, 2020
Export Citation:
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Assignee:
MICROBIOPHARM JAPAN CO LTD (JP)
International Classes:
C12P1/00; C12N1/15; C12N1/19; C12N1/21; C12N9/18; C12N15/55
Attorney, Agent or Firm:
SIKs & Co. (JP)
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