Title:
METHOD OF EXTENDING LONG−CHAIN DNA
Document Type and Number:
WIPO Patent Application WO/2002/097083
Kind Code:
A1
Abstract:
A method of extending a long−chain nucleic acid by using a gel and a low frequency AC electric field which makes it possible to conveniently and quickly collect and re−use an extended and analyzed long−chain DNA&semi nucleic acid molecules extended by this method&semi and a method of extending nucleic acid molecules involving the step of providing the nucleic acid molecules in a gel and the step of applying a low−frequency AC electric field to the nucleic acid molecules in the gel.
Inventors:
KAJI NORITADA (JP)
UEDA MASANORI (JP)
BABA YOSHINOBU (JP)
UEDA MASANORI (JP)
BABA YOSHINOBU (JP)
Application Number:
PCT/JP2001/007522
Publication Date:
December 05, 2002
Filing Date:
August 31, 2001
Export Citation:
Assignee:
JAPAN SCIENCE & TECH CORP (JP)
KAJI NORITADA (JP)
UEDA MASANORI (JP)
BABA YOSHINOBU (JP)
KAJI NORITADA (JP)
UEDA MASANORI (JP)
BABA YOSHINOBU (JP)
International Classes:
C12N15/09; G01N27/447; (IPC1-7): C12N15/00; C12M1/00; G01N27/26; G01N33/50
Foreign References:
| JPH05317047A | 1993-12-03 | |||
| JPH11206373A | 1999-08-03 | |||
| JPH08322569A | 1996-12-10 |
Other References:
Masao WASHAZU, "DNA no Bunshiryou wo Nagasa de hakaru", Saibou Kougaku, (1989), Vol. 8, No. 11, pages 1011 to 1015.
Masanori UEDA, "Genshi-kan ryoku Kenbikyou (AFM) ni yoru Tan-itsu DNA Bunshi-nai no Bunshi-kanryoku Keisaku-hou no Kaihatsu", Shimazu Kagaku Gijutsu Shinkou Zaidan Kenkyuu Houkoku, (2000), Vol. 1998, pages 11 to 17.
Ronald M. Kantor et al., "Dynamics of DNA Molecules in Gel Studied by Fluorescence Microscopy", Biochemical and Biophysical Research Communications, March 1999, Vol. 258, No. 1, pages 102 to 108.
Thomas A. J. Duke et al., "Pulsed-field electrophoresis in microlithographic arrays", Electrophoresis, (1996), Vol. 17, No. 6, pages 1075 to 1079.
Masanori UEDA, "Genshi-kan ryoku Kenbikyou (AFM) ni yoru Tan-itsu DNA Bunshi-nai no Bunshi-kanryoku Keisaku-hou no Kaihatsu", Shimazu Kagaku Gijutsu Shinkou Zaidan Kenkyuu Houkoku, (2000), Vol. 1998, pages 11 to 17.
Ronald M. Kantor et al., "Dynamics of DNA Molecules in Gel Studied by Fluorescence Microscopy", Biochemical and Biophysical Research Communications, March 1999, Vol. 258, No. 1, pages 102 to 108.
Thomas A. J. Duke et al., "Pulsed-field electrophoresis in microlithographic arrays", Electrophoresis, (1996), Vol. 17, No. 6, pages 1075 to 1079.
Attorney, Agent or Firm:
Hosoda, Yoshinori c/o Hosoda International Patent Office OMM Building 5th Floor P.O. Box 26 7-31 (Otemae 1-chome Chuo-ku Osaka-shi, Osaka, JP)
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