Title:
METHOD FOR FLUORESCENT LABELING OF PROTEIN
Document Type and Number:
WIPO Patent Application WO/2012/105596
Kind Code:
A1
Abstract:
[Problem] To provide a method for the fluorescent labeling of a protein that can even be used for the fluorescent labeling of a biological molecule (protein) in vivo. [Solution] A method for the fluorescent labeling of a target protein, comprising a step for the intracellular formation of a fused protein of the protein to be labeled and a mutant β-lactamase, a step for using intracellular esterase for the conversion of a compound represented by the following formula (I) or a salt thereof to a compound represented by the following formula (II) or a salt thereof, and a step for the reaction of the fused protein and the compound represented by formula (II) or a salt thereof.
Inventors:
KIKUCHI KAZUYA
MIZUKAMI SHIN
WATANABE SHUJI
AKIMOTO YURI
MIZUKAMI SHIN
WATANABE SHUJI
AKIMOTO YURI
Application Number:
PCT/JP2012/052227
Publication Date:
August 09, 2012
Filing Date:
February 01, 2012
Export Citation:
Assignee:
UNIV OSAKA (JP)
KIKUCHI KAZUYA
MIZUKAMI SHIN
WATANABE SHUJI
AKIMOTO YURI
KIKUCHI KAZUYA
MIZUKAMI SHIN
WATANABE SHUJI
AKIMOTO YURI
International Classes:
C07K1/13; C07D499/04; C07D499/74; C07K19/00; C12N15/09; G01N33/533; G01N33/58
Foreign References:
| JP2010119382A | 2010-06-03 | |||
| US20070105182A1 | 2007-05-10 |
Other References:
KAZUYA KIKUCHI ET AL.: "Bioimaging no Tameno Shinki Zn2+ Keiko Probe", KAGAKU TO SEIBUTSU, vol. 39, 2001, pages 549 - 553
KAZUYA KIKUCHI: "Seisaibo Keiko Probe o Mochiita Seitai Kashika Kaiseki", NEWS LETTER, vol. 16, 2001, pages 4 - 7
KAZUYA KIKUCHI: "Kinoteki Keiko Hyoshiki Donyu ni yoru Amyloid Chikuseki Katei no Bunseki Shuho Kaihatsu ni Kansuru Kenkyu", AMYLOSPHEROID KASETSU NI YORU ALZHEIMER'S DISEASE BYOTAI KAIMEI TO RINSHO OYO NI KANSURU KENKYU HEISEI 18 NENDO SOKATSU BUNTAN KENKYU HOKOKUSHO KOTO DOBUTSU MODEL KOCHIKU TO SEITAI REAL TIME KANSOKUHO KAIHATSU NI YORU APPROACH, 2007, pages 12 - 13
YURI AKOMOTO ET AL.: "Hen'itai beta-Lactamase o Mochiita Saibonai Tanpakushitsu Label-ka Ho no Kaihatsu", THE 91ST ANNUAL MEETING OF THE CHEMICAL SOCIETY OF JAPAN IN SPRING (2011) KOEN YOKOSHU, vol. III, 11 March 2011 (2011-03-11), pages 718
KAZUYA KIKUCHI: "Seisaibo Keiko Probe o Mochiita Seitai Kashika Kaiseki", NEWS LETTER, vol. 16, 2001, pages 4 - 7
KAZUYA KIKUCHI: "Kinoteki Keiko Hyoshiki Donyu ni yoru Amyloid Chikuseki Katei no Bunseki Shuho Kaihatsu ni Kansuru Kenkyu", AMYLOSPHEROID KASETSU NI YORU ALZHEIMER'S DISEASE BYOTAI KAIMEI TO RINSHO OYO NI KANSURU KENKYU HEISEI 18 NENDO SOKATSU BUNTAN KENKYU HOKOKUSHO KOTO DOBUTSU MODEL KOCHIKU TO SEITAI REAL TIME KANSOKUHO KAIHATSU NI YORU APPROACH, 2007, pages 12 - 13
YURI AKOMOTO ET AL.: "Hen'itai beta-Lactamase o Mochiita Saibonai Tanpakushitsu Label-ka Ho no Kaihatsu", THE 91ST ANNUAL MEETING OF THE CHEMICAL SOCIETY OF JAPAN IN SPRING (2011) KOEN YOKOSHU, vol. III, 11 March 2011 (2011-03-11), pages 718
Attorney, Agent or Firm:
IKEUCHI SATO & PARTNER PATENT ATTORNEYS (JP)
Patent business corporation Ikeuchi and Sato and partners (JP)
Patent business corporation Ikeuchi and Sato and partners (JP)
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