Title:
METHOD FOR INDUCING DIFFERENTIATION OF PERITONEAL MESOTHELIUM CELLS FROM PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2022/181776
Kind Code:
A1
Abstract:
The present invention addresses the problem of inducing, from pluripotent stem cells, cells that are functional similar to PMC. Said problem is solved by a method that is for inducing peritoneal mesothelium cells from pluripotent stem cells and that comprises: a first culturing step for culturing pluripotent stem cells in the presence of a basic fibroblast growth factor and a bone morphogenetic factor; a second culturing step for culturing the cells cultured in the first culturing step, in the presence of the bone morphogenetic factor but at a concentration higher than that in the first culturing step, and the basic fibroblast growth factor; and a third culturing step for culturing the cells cultured in the second culturing step in the presence of a vitamin-A compound, a glycogen synthase kinase-3 inhibitor, and an inhibitor of activin kinase-2 and activin kinase-3.
Inventors:
KATO TADASHI (JP)
HITOMI HIROFUMI (JP)
HITOMI HIROFUMI (JP)
Application Number:
PCT/JP2022/007960
Publication Date:
September 01, 2022
Filing Date:
February 25, 2022
Export Citation:
Assignee:
KANSAI MEDICAL UNIV EDUCATIONAL CORPORATION (JP)
International Classes:
C12N5/071; C12N5/10
Domestic Patent References:
| WO2015178498A1 | 2015-11-26 | |||
| WO2014115776A1 | 2014-07-31 | |||
| WO2016031824A1 | 2016-03-03 | |||
| WO2018139548A1 | 2018-08-02 |
Other References:
NATSUKO F. INAGAKI; FUYUKI F. INAGAKI; NORIHIRO KOKUDO; ATSUSHI MIYAJIMA: "Generation of mesothelial progenitor‐like cells from mouse‐induced pluripotent stem cells", FEBS LETTERS, ELSEVIER, AMSTERDAM., NL, vol. 593, no. 4, 18 January 2019 (2019-01-18), NL , pages 386 - 394, XP071256913, ISSN: 0014-5793, DOI: 10.1002/1873-3468.13325
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
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