METHOD FOR ISOLATING EXOSOME AND EXOSOME ISOLATION KIT

Title:

METHOD FOR ISOLATING EXOSOME AND EXOSOME ISOLATION KIT

Document Type and Number:

WIPO Patent Application WO/2019/039179

Kind Code:

A1

Abstract:

Provided is a means for conveniently isolating exosome from a sample containing exosome. The present invention relates to a method for isolating exosome, the method comprising: a complex formation step for binding a specific peptide containing lysine carried on a carrier and exosome in a sample to form a complex; and a dissociation step for dissociating the exosome from the complex by contacting the complex with a dissociation buffer containing metal cations.

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Inventors:

KURODA AKIO (JP)
ISHIDA TAKENORI (JP)

Application Number:

PCT/JP2018/027945

Publication Date:

February 28, 2019

Filing Date:

July 25, 2018

Export Citation:

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Assignee:

UNIV HIROSHIMA (JP)

International Classes:

G01N33/48; C07K4/00; C07K7/08; C07K14/00

Domestic Patent References:

WO2014168230A1

2014-10-16

Foreign References:

JP2017038566A	2017-02-23
JP2017044674A	2017-03-02

Other References:

GHOSH, A. ET AL.: "Rapid Isolation of Extracellular Vesicles from Cell Culture and Biological Fluids Using a Synthetic Peptide with Specific Affinity for Heat Shock Proteins", PLOS ONE, vol. 9, no. 10/e110443, 17 October 2014 (2014-10-17), pages 1 - 12, XP055286213
"Master Lesson of Exosome Handling", 2014, YODOSHA CO., LTD.
"Experimental Medicine (Jikken Igaku)", vol. 34, 2016, pages: 1390 - 1396
See also references of EP 3674704A4