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Title:
METHOD AND MATERIALS FOR SEPARATING NUCLEIC ACID MATERIALS
Document Type and Number:
WIPO Patent Application WO/2011/081869
Kind Code:
A3
Abstract:
Nucleic acid material can be effectively separated from a fluid by first contacting the fluid with a positively charged polymer which binds the nucleic acid material. Thereafter, the polymer, having the nucleic acid material bonded thereto, is contacted with a releasing agent which comprises a solution of an alkaline material and a glycol. The solution has a pH of no more than 12 and operates to release the nucleic acid material from the polymer under relatively low temperature conditions, typically no more than 5O0C, and in particular instances, no more than 4O0C. The glycol material may comprise a monomeric glycol such as ethylene glycol, propylene glycol, or the like, or it may comprise a polymeric glycol such as polyethylene glycol. Also disclosed is a novel positively charged polymer which may be employed in the separation process. This polymer comprises an acidified polyamine, such as polyethyleneimine which has been reacted with a nonacidified polyethyleneimine in a coupling reaction. The acidified polyethyleneimine may be a coarboxylated and/or sulfonated polyethyleneimine.

Inventors:
WU BETTY (US)
Application Number:
PCT/US2010/060025
Publication Date:
October 13, 2011
Filing Date:
December 13, 2010
Export Citation:
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Assignee:
WU BETTY (US)
International Classes:
C12Q1/68; C12N15/10; C12S3/20
Foreign References:
US6783962B12004-08-31
US20060234251A12006-10-19
US20090011469A12009-01-08
US20090068662A12009-03-12
Other References:
See also references of EP 2513335A4
Attorney, Agent or Firm:
CITKOWSKI, Ronald, W. et al. (KrassSprinkle, Anderson & Citkowski, P.C.,2701 Troy Center Drive, Suite 330,Post Office Box 702, Troy MI, US)
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