Title:
METHOD FOR MEASURING EFFECTIVE DIFFUSION COEFFICIENT OF BIOMOLECULE
Document Type and Number:
WIPO Patent Application WO/2020/069640
Kind Code:
A1
Abstract:
Provided is a method for measuring the effective diffusion coefficient of a biomolecule, comprising the following steps: A1: planning an initial label region ROI0 of a biomolecule whose diffusion coefficient is to be measured; planning a diffusion process image collection region ROI1; A2: performing fluorescent or other optical labeling of the biomolecule in the region ROI0; A3: diffusing the biomolecule in ROI0 and ensuring that the diffusion area of the biomolecule is smaller than the region ROI1, and performing dynamic image collection on the biomolecular diffusion process in the region ROI1; A4: checking whether the collected dynamic image is valid; A5: performing dimensionality reduction on the image data, and generating planar coordinate data of the biomolecule diffusion process according to the image data; A6: according to the generated planar coordinate data, using a formula to derive the effective diffusion coefficient of the biomolecular diffusion process; there are few assumptions regarding applications, the range of applications is broad.
More Like This:
| WO/2002/014876 | TEST UNIT AND METHOD FOR THE PRODUCTION OF STABLE FORMULATIONS |
| JPH11500075 | It gets wet for a complex and displays. |
| JP2001116676 | DIFFUSION TESTING DEVICE |
Inventors:
NI SHIWEI (CN)
ZHANG XI (CN)
YANG YUFENG (CN)
ZHANG XI (CN)
YANG YUFENG (CN)
Application Number:
PCT/CN2018/124270
Publication Date:
April 09, 2020
Filing Date:
December 27, 2018
Export Citation:
Assignee:
UNIV FU ZHOU (CN)
International Classes:
G01N13/00; G01N33/58
Domestic Patent References:
| WO2018048063A1 | 2018-03-15 |
Foreign References:
| JP2005043278A | 2005-02-17 | |||
| CN107121362A | 2017-09-01 | |||
| US20150132775A1 | 2015-05-14 | |||
| US20160116468A1 | 2016-04-28 |
Other References:
DONG, SHIQING ET AL.: "Measuring the diffusion coefficient of ganglioside on cell membrane by fluorescence correlation spectroscopy", IOP CONF. SERIES: JOURNAL OF PHYSICS: CONF. SERIES, 31 December 2017 (2017-12-31), pages 1 - 5, XP055698914, DOI: 10.1088/1742-6596/844/1/012047
Attorney, Agent or Firm:
FUZHOU YUANCHUANG PATENT AND TRADEMARK AGENT LTD. (CN)
Download PDF: