Title:
METHOD FOR PERFORMING FLUORESCENCE ASSAY IN CELL-FREE PROTEIN SYNTHESIS ENVIRONMENT
Document Type and Number:
WIPO Patent Application WO/2021/184651
Kind Code:
A1
Abstract:
A method for performing fluorescence assay in a cell-free protein synthesis environment. The method comprises the following steps: providing a multi-well plate (200) comprising a cover plate (160) and a base (110) provided with a plurality of wells (100), the wells (100) being formed by one or more side walls (130), a bottom part II (140) and an opening (150), the cover plate (160) matching the opening (150), the volume of a reaction cavity (120) of the wells (100) being less than 20 μL, and some wells (100) of the plurality of wells (100) being in communication with each other; providing a fluid (170) in some of the wells(100), and when the fluid (170) is a cell-free reaction mixture or a fluorescent assay material, adding a biochemical factor and one or more of a template DNA, a template RNA, an additive and a reaction auxiliary factor; when the fluid (170) is a cell-free reaction mixture, a fluorescent assay material and a biochemical factor, adding one or more of a template DNA, a template RNA, an additive and a reaction auxiliary factor; placing the cover plate (160) on top of the base (110), the fluid (170) being in contact with both the bottom II (140) of the wells (100) and the cover plate (160); and incubating the multi-well plate (200) for a period of time. The method for performing fluorescent assay in a cell-free protein synthesis environment can reduce reagent and assay costs.
Inventors:
KAI LEI (CN)
Application Number:
PCT/CN2020/107648
Publication Date:
September 23, 2021
Filing Date:
August 07, 2020
Export Citation:
Assignee:
JIANGSU ZHIDIAN BIOTECHNOLOGY CO LTD (CN)
International Classes:
G01N21/64
Foreign References:
| CN111366566A | 2020-07-03 | |||
| EP1211514A1 | 2002-06-05 | |||
| CN102277294A | 2011-12-14 | |||
| CN106153891A | 2016-11-23 | |||
| CN109750057A | 2019-05-14 | |||
| US20020142387A1 | 2002-10-03 | |||
| EP1736781A1 | 2006-12-27 |
Attorney, Agent or Firm:
BEIJING HUAIHAI INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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