Title:
METHOD FOR PREPARING L-GLUFOSINATE USING BIOLOGICAL MULTI-ENZYME COUPLING
Document Type and Number:
WIPO Patent Application WO/2022/127887
Kind Code:
A1
Abstract:
The present application relates to a method for preparing L-glufosinate using biological multi-enzyme coupling, comprising: in the presence of (R)-transaminase, (S)-transaminase, an amino acceptor, and an amino donor, converting D,L-glufosinate into L-glufosinate. The present method may achieve an efficient splitting of high-concentration D,L-glufosinate to prepare L-glufosinate.
Inventors:
WANG HUALEI (CN)
WEI DONGZHI (CN)
WU CHENGJUN (CN)
LIU QINGHAI (CN)
LUO ZHONGHUA (CN)
ZHANG CHANGLEI (CN)
WEI DONGZHI (CN)
WU CHENGJUN (CN)
LIU QINGHAI (CN)
LUO ZHONGHUA (CN)
ZHANG CHANGLEI (CN)
Application Number:
PCT/CN2021/139010
Publication Date:
June 23, 2022
Filing Date:
December 17, 2021
Export Citation:
Assignee:
YONGNONG BIOSCIENCES CO LTD (CN)
UNIV EAST CHINA SCIENCE & TECH (CN)
NINGXIA YONGNONG BIOSCIENCES CO LTD (CN)
UNIV EAST CHINA SCIENCE & TECH (CN)
NINGXIA YONGNONG BIOSCIENCES CO LTD (CN)
International Classes:
C12P13/04
Foreign References:
| US5587319A | 1996-12-24 | |||
| CN101384723A | 2009-03-11 | |||
| CN112410383A | 2021-02-26 | |||
| CN111321193A | 2020-06-23 |
Other References:
LOU, YI-YUAN ET AL.: "Progresses in Biosynthesis of L-Phosphinothricin", MODERN AGROCHEMICALS, vol. 8, no. 3, 10 June 2009 (2009-06-10), pages 1 - 4,10, XP055942601
BARTSCH K. ET AL.: "Stereospecific production of the herbicide Phosphothricin (Glufosinate): purification of Aspartate Transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 62, no. 10, 1 October 1996 (1996-10-01), US , pages 3794 - 3799, XP002144376, ISSN: 0099-2240
BARTSCH K. ET AL.: "Stereospecific production of the herbicide Phosphothricin (Glufosinate): purification of Aspartate Transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 62, no. 10, 1 October 1996 (1996-10-01), US , pages 3794 - 3799, XP002144376, ISSN: 0099-2240
Attorney, Agent or Firm:
ZHONG LUN LAW FIRM (CN)
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