Title:
METHOD FOR PREPARING RECOMBINANT ACREMONIUM CHRYSOGENUM STRAIN PRODUCING HIGH CONCENTRATION OF DEACETOXYCEPHALOSPROIN C FOR PREPARATION OF 7-ADCA, AND STRAIN PREPARED BY SAME METHOD
Document Type and Number:
WIPO Patent Application WO/2021/054545
Kind Code:
A1
Abstract:
The present invention relates to a method for preparing a recombinant acremonium chrysogenum strain producing a high concentration of deacetoxycephalosporin C (DAOC), and a strain prepared by same method and, more specifically, to a method for preparing an acremonium chrysogenum strain producing DAOC with high yield, a recombinant acremonium chrysogenum strain prepared by same method, a DAOC production method for culturing the strain under conditions capable of producing DAOC and recovering the DAOC, a polynucleotide for high DAOC expression in the acremonium chrysogenum strain used in the production method, an expression vector for high DAOC expression in the acremonium chrysogenum strain, and a method for producing 7-ADCA by treating the produced DAOC with CPC acylase.
Inventors:
SHIN YONG CHUL (KR)
PIAO ZHE (KR)
YOON SANG HWAL (KR)
LEE HYEON SEO (KR)
KANG MI SUK (KR)
PIAO XUEMEI (KR)
KIM YOU MI (KR)
LI HONG XIAN (KR)
JEONG DONG WON (KR)
SHIM JAE MIN (KR)
JUNG JANG HYUN (KR)
SEO DONG IL (KR)
KIM SEUNG KI (KR)
PIAO ZHE (KR)
YOON SANG HWAL (KR)
LEE HYEON SEO (KR)
KANG MI SUK (KR)
PIAO XUEMEI (KR)
KIM YOU MI (KR)
LI HONG XIAN (KR)
JEONG DONG WON (KR)
SHIM JAE MIN (KR)
JUNG JANG HYUN (KR)
SEO DONG IL (KR)
KIM SEUNG KI (KR)
Application Number:
PCT/KR2020/001711
Publication Date:
March 25, 2021
Filing Date:
February 06, 2020
Export Citation:
Assignee:
AMICOGEN INC (KR)
International Classes:
C12N9/10; C12N9/02; C12P35/00
Domestic Patent References:
| WO1998002567A2 | 1998-01-22 |
Foreign References:
| KR101808192B1 | 2018-01-18 | |||
| KR101728906B1 | 2017-04-20 | |||
| KR20020022701A | 2002-03-27 |
Other References:
VELASCO, J. ET AL.: "Environmentally safe production of 7-aminodeacetoxycephalosporanic acid (7-ADCA) using recombinant strains of Acremonium chrysogenum", NATURE BIOTECHNOLOGY, vol. 18, 2000, pages 857 - 861, XP055808068
HU, Y ET AL.: "Study on genetic engineering of Acremonium chrysogenum, the cephalosporin C producer", SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, vol. 1, 2016, pages 143 - 149, XP055808069
HU, Y ET AL.: "Study on genetic engineering of Acremonium chrysogenum, the cephalosporin C producer", SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, vol. 1, 2016, pages 143 - 149, XP055808069
Attorney, Agent or Firm:
LEE, Hee Sook (KR)
Download PDF: