Title:
A METHOD OF PREPARING RECOMBINANT STREPTOKINASE
Document Type and Number:
WIPO Patent Application WO/1995/027050
Kind Code:
A1
Abstract:
The present invention relates to constructing expression plasmid pSTE-SK-1 by using streptokinase vectors, such as pLY-4, and transforming E. coli with such plasmid, then inducing the expression of the r-SK gene by heating. The engineered bacteria are propagated by fermenting and destructed, collected inclusion body by centrifugalizing the r-SK product is purified via a two-step method after renaturation. The production and purity of the invention are high and cost low.
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Inventors:
SONG HOUYAN (CN)
Application Number:
PCT/CN1995/000024
Publication Date:
October 12, 1995
Filing Date:
April 03, 1995
Export Citation:
Assignee:
SHANGHAI MEDICAL UNIVERSITY (CN)
SONG HOUYAN (CN)
SONG HOUYAN (CN)
International Classes:
C07K14/315; C12N9/70; A61K38/00; (IPC1-7): C12N9/70
Foreign References:
| US5296366A | 1994-03-22 | |||
| US5066589A | 1991-11-19 | |||
| EP0151337A2 | 1985-08-14 | |||
| EP0489201A1 | 1992-06-10 | |||
| EP0407942A2 | 1991-01-16 |
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