Title:
METHOD FOR PREPARING (S)-1,2,3,4-TETRAHYDROISOQUINOLINE-1-CARBOXYLIC ACID AND DERIVATIVE THEREOF BY USING ENZYMATIC RESOLUTION
Document Type and Number:
WIPO Patent Application WO/2019/128387
Kind Code:
A1
Abstract:
The present invention provides a method for preparing (S)-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid and a derivative thereof by using enzymatic resolution, the method comprising the step of: using racemic 1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid (1) or racemic 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid (2) as a substrate and using an ex vivo D-amino acid oxidase or a cell intracellularly expressing D-amino acid oxidase as a catalyst to selectively catalyze the oxidative dehydrogenation reaction of (R)-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid or (R)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid, (S)-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid or (S)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid being not catalyzed and remaining in the reaction system. The method achieves a conversion of greater than 49%, and an ee value of greater than 99%.
Inventors:
WU JIANPING (CN)
JU SHUYUN (CN)
SHI JUNWEI (CN)
QIAN MINGXI (CN)
YANG LIRONG (CN)
JU SHUYUN (CN)
SHI JUNWEI (CN)
QIAN MINGXI (CN)
YANG LIRONG (CN)
Application Number:
PCT/CN2018/110179
Publication Date:
July 04, 2019
Filing Date:
October 15, 2018
Export Citation:
Assignee:
TONGLI BIOMEDICAL CO LTD (CN)
UNIV ZHEJIANG (CN)
UNIV ZHEJIANG (CN)
International Classes:
C12P41/00; C12P17/12
Foreign References:
| CN104557911A | 2015-04-29 | |||
| CN1922328A | 2007-02-28 | |||
| CN101194020A | 2008-06-04 |
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT & TRADEMARK AGENCY LTD. (CN)
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