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Patent Searching and Data


Title:
METHOD FOR PREPARING SERINE PROTEASE
Document Type and Number:
WIPO Patent Application WO/2022/143186
Kind Code:
A1
Abstract:
Provided are an expression vector and host cell for co-expressing a serine protease and a serine protease inhibitor, and a method for preparing a serine protease by means of the expression vector or host cell. The serine protease inhibitor inhibits partial activity of an overexpressed serine protease, reduces the cytotoxicity of the serine protease on an expression cell line, and improves the tolerance of the expression cell line on the serine protease with the same expression amount.

Inventors:
WANG DINGLI (CN)
Application Number:
PCT/CN2021/138653
Publication Date:
July 07, 2022
Filing Date:
December 16, 2021
Export Citation:
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Assignee:
FOSHAN CANTON BIOLOGICS CO LTD (CN)
CANTON BIOLOGICS CO LTD (CN)
PROGENBIO CO LTD (CN)
International Classes:
C12N15/81; C07K14/81; C12N1/19; C12N9/64; C12N15/67; C12P21/02
Foreign References:
US5648254A1997-07-15
CN110770340A2020-02-07
IN201947051091A2019-12-20
CN102388135A2012-03-21
Other References:
TSUZUKI SATOSHI, MURAI NOBUHITO, MIYAKE YUKA, INOUYE KUNIYO, HIRAYASU HIROFUMI, IWANAGA TOSHIHIKO, FUSHIKI TOHRU: "Evidence for the occurrence of membrane-type serine protease 1/matriptase on the basolateral sides of enterocytes", BIOCHEMICAL JOURNAL, PUBLISHED BY PORTLAND PRESS ON BEHALF OF THE BIOCHEMICAL SOCIETY., GB, vol. 388, no. PART 2, 1 June 2005 (2005-06-01), GB , pages 679 - 687, XP008103674, ISSN: 0264-6021, DOI: 10.1042/BJ20041639
S├śREN E. DEGN, THIEL STEFFEN, JENSENIUS JENS C.: "Recombinant expression of the autocatalytic complement protease MASP-1 is crucially dependent on co-expression with its inhibitor, C1 inhibitor", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA., vol. 88, no. 2, 1 April 2013 (2013-04-01), SAN DIEGO, CA. , pages 173 - 182, XP055402258, ISSN: 1046-5928, DOI: 10.1016/j.pep.2013.01.002
Attorney, Agent or Firm:
IPSUNNY (CN)
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