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Patent Searching and Data


Title:
METHOD FOR PREPARING SINGLE-STRANDED DNA LIBRARIES
Document Type and Number:
WIPO Patent Application WO2004070007
Kind Code:
A3
Abstract:
This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.

Inventors:
COSTA GINA L (US)
WEINER MICHAEL P (US)
LEAMON JOHN (US)
ROTHBERG JONATHAN (US)
Application Number:
PCT/US2004/002571
Publication Date:
December 22, 2005
Filing Date:
January 28, 2004
Export Citation:
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Assignee:
454 CORP (US)
COSTA GINA L (US)
WEINER MICHAEL P (US)
LEAMON JOHN (US)
ROTHBERG JONATHAN (US)
International Classes:
B01L3/00; C07H21/00; C12N15/10; C12P19/34; G01N21/25; G01N21/64; (IPC1-7): C12Q1/68
Foreign References:
US6270966B12001-08-07
US6004783A1999-12-21
US6060245A2000-05-09
Other References:
HUKTMAN T. AND UHLEN M.: "Solid-phase cloning to create sublibraries suitable for DNA sequencing", JOURNAL OF BIOTECHNOLOGY, vol. 35, 1994, pages 229 - 238, XP002931121
See also references of EP 1594950A4
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