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Title:
METHOD FOR PRODUCING CULTURED TISSUE, AND PREPARATION FOR EXTERNAL APPLICATION
Document Type and Number:
WIPO Patent Application WO/2021/039833
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a novel means for preventing the inhibition of the proliferation of keratinocytes even when the keratinocytes begin to contact with each other. The present invention can solve the problem by culturing keratinocytes while bringing the keratinocytes into contact with a common culture medium with fibroblasts that have been treated with a composition containing a specific compound.

Inventors:
MUTO JUN (JP)
SAYAMA KOJI (JP)
Application Number:
PCT/JP2020/032153
Publication Date:
March 04, 2021
Filing Date:
August 26, 2020
Export Citation:
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Assignee:
UNIV NAT CORP EHIME UNIV (JP)
International Classes:
A61P1/00; A61K31/7016; A61P1/02; A61P11/02; A61P15/02; A61P17/02; A61P27/02; A61P27/16; A61P43/00; C12N5/071; C07H3/04
Domestic Patent References:
WO2007029676A12007-03-15
WO2005087286A12005-09-22
WO2005087286A12005-09-22
Foreign References:
JP2010172247A2010-08-12
JP2010172247A2010-08-12
JP2773058B21998-07-09
JPH07143876A1995-06-06
JPH0873504A1996-03-19
JP3182679B22001-07-03
JP2000228980A2000-08-22
JP3066624B22000-07-17
JP2012205516A2012-10-25
Other References:
OZEK, D., ET AL. ET AL.: "Effect of the bioprotectant agent trehalose on corneal epithelial healing after corneal cross-linking for keratoconus", ARQ. BRAS. OFTALMOL., vol. 81, no. 6, 2018, pages 505 - 509, XP055796442, ISSN: 0004-2749
TAKEUCHI, K. ET AL.: "Inhibitory effects of trehalose on fibroblast proliferation and implications for ocular surgery", EXPERIMENTAL EYE RESEARCH, vol. 91, 2010, pages 567 - 577, XP027428493, ISSN: 0014- 4835
HASHIMOTO ET AL., CELL TISSUE RES, vol. 69, 2006, pages 326
INOUE ET AL., ORGAN BIOLOGY (JPN, vol. 22, no. 1, 2015, pages 57 - 65
See also references of EP 4023289A4
Attorney, Agent or Firm:
UNIUS PATENT ATTORNEYS OFFICE (JP)
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