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Title:
METHOD FOR PRODUCING MONOVALENT CCAP PRODUCT
Document Type and Number:
WIPO Patent Application WO/2021/080008
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an Fc-modified antibody which has a long half-life in the blood and is obtained through a CCAP method. More specifically, the purpose of the present invention is to provide an antibody in which IgBP is bound to only one site, on the basis of the findings found by the present inventors. The present invention provides a target antibody or the like by purifying and producing an Fc-modified antibody or the like using a column that binds to an Fc region of an antibody. Specifically, the present invention provides an antibody in which only one site among two binding sites of Fc is selectively modified by adsorbing an IgBP-binding antibody produced through the CCAP method on a carrier of a IgBP-immobilized column, or by adding, in a state where only one Fc of the antibody is bound to an IgBP-binding column, a peptide reagent for CCAP to the column and performing the reaction of the CCAP method in the column.

Inventors:
ITO YUJI (JP)
TAKAHASHI NOBUAKI (JP)
NAKANO RYOSUKE (JP)
MAEDA SAYAKA (JP)
Application Number:
PCT/JP2020/039978
Publication Date:
April 29, 2021
Filing Date:
October 23, 2020
Export Citation:
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Assignee:
UNIV KAGOSHIMA (JP)
International Classes:
A61K39/395; A61K47/64; A61K51/10; C07K1/22; C07K7/08; C07K16/00; C07K19/00; C12N11/00
Domestic Patent References:
WO2018092867A12018-05-24
WO2013027796A12013-02-28
WO2018230257A12018-12-20
WO2016186206A12016-11-24
WO2016186206A12016-11-24
WO2018230257A12018-12-20
WO2008054030A12008-05-08
WO2013027796A12013-02-28
WO2018199337A12018-11-01
Other References:
MUGURUMA KYOHEI, ITO MAYU, FUKUDA AKANE, KISHIMOTO SATOSHI, TAGUCHI AKIHIRO, TAKAYAMA KENTARO, TANIGUCHI ATSUHIKO, ITO YUJI, HAYAS: "Synthesis and structure- activity relationship studies of lgG-binding peptides focused on the C-terlllinal histidine residue", MEDCHEMCOMM, vol. 10, 1 August 2019 (2019-08-01), pages 1789 - 1795, XP055820635
MUGURUMA KYOHEI, FUJITA KONOMI, FUKUDA AKANE, KISHIMOTO SATOSHI, SAKAMOTO SOICHIRO, ARIMA RISAKO, ITO MAYU, KAWASAKI MAYU, NAKANO : "Kinetics-Based Structural Requirements of HU 111an Immunoglobulin G Binding Peptides", ACS OMEGA, vol. 4, 28 August 2019 (2019-08-28), pages 14390 - 14397, XP055820637
KISHIMOTO, S. ET AL., BIOCONJUG. CHEM., vol. 30, 2019, pages 698 - 702
KYOHEIMUGURUMA, ACS OMEGA, vol. 4, no. 11, 2019, pages 14390 - 14397
KEI YAMADA, ANGEW. CHEM., vol. 131, 2019, pages 5648 - 5653
BRAISTED, A.C., PROC. NATL. ACAD. SCI. U.S.A., vol. 93, 1996, pages 5688 - 92
Attorney, Agent or Firm:
NAGOYA INTERNATIONAL PATENT FIRM et al. (JP)
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