Title:
METHOD FOR PRODUCING PHOSPHOLIPID-CONTAINING COMPOSITION, AND PHOSPHOLIPID-CONTAINING COMPOSITION
Document Type and Number:
WIPO Patent Application WO/2013/187328
Kind Code:
A1
Abstract:
A method for producing a phospholipid-containing composition that contains phosphatidylserine in an amount of 10 wt% or more relative to the whole weight of the phospholipid-containing composition and has a highly unsaturated fatty acid content of 10 to 40 wt% relative to the total amount of constituent fatty acids, said method comprising steps (1) and (2) and steps (3) and (4) as mentioned below. The method enables a phospholipid-containing composition that contains phosphatidylserine having many highly unsaturated fatty acid molecules bound to position-2 thereof to be supplied at low cost and in a steady manner. Step (1): an esterification reaction between a lysophospholipid and a highly unsaturated fatty acid with phospholipase A2(PLA2) is carried out to produce a phospholipid; step (2): subsequent to step (1), the activity of PLA2 in the phospholipid is reduced to 10 U/g (phospholipid) or less; step (3): a base exchange reaction in a mixture of the phospholipid and serine is carried out in the presence of phospholipase D (PLD) to produce a phospholipid-containing composition that contains phosphatidylserine; and step (4): the composition is separated.
Inventors:
SHIGEEDA MAYUMI (JP)
TANAKA TATSUSHI (JP)
INOUE YOSHIKAZU (JP)
TANAKA TATSUSHI (JP)
INOUE YOSHIKAZU (JP)
Application Number:
PCT/JP2013/065853
Publication Date:
December 19, 2013
Filing Date:
June 07, 2013
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12P9/00; C12P7/64
Domestic Patent References:
WO2011004794A1 | 2011-01-13 |
Foreign References:
JP2010068799A | 2010-04-02 | |||
JPS63233750A | 1988-09-29 | |||
JPH09121879A | 1997-05-13 | |||
JP2001186898A | 2001-07-10 | |||
JP2004215528A | 2004-08-05 | |||
JP2012249597A | 2012-12-20 | |||
JPH09121879A | 1997-05-13 | |||
JP2011525525A | 2011-09-22 | |||
JP2010068799A | 2010-04-02 |
Other References:
JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY, vol. 13, 1990, pages 126 - 129
See also references of EP 2862937A4
See also references of EP 2862937A4
Attorney, Agent or Firm:
YANAGINO Takao et al. (JP)
Takao Yanagino (JP)
Takao Yanagino (JP)
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