Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
METHOD FOR PRODUCING REGENERATED T CELLS VIA IPS CELLS
Document Type and Number:
WIPO Patent Application WO/2022/059780
Kind Code:
A1
Abstract:
[Problem] To provide a method for producing regenerated T cells by introducing antigen-specific T cell receptors to iPS cell clones or hematopoietic stem cells differentiated from iPS cell clones, immature T cells or mature T cells. [Solution] The present invention is a method for producing regenerated T cells via iPS cells, the method comprising: (1) a step for obtaining T cells from a subject, and preparing, from individual cells in a group of T cells that is reactive to a tumor-related antigen, cDNAs that code for TCRα chain and TCRβ chain; (2) a step for initializing the subject-derived peripheral blood mononuclear cells from which B cells and T cells have been removed, or T cells, to iPS cells, and selecting, from the iPS cells thus obtained, iPS cell clones that demonstrate high differentiation efficiency into T cells; (3) a step for introducing the cDNAs from the individual cells into the iPS cell clones, into hematopoietic stem cells differentiated from the iPS cell clones, into immature T cells differentiated from the hematopoietic stem cells, or into mature T cells differentiated from the immature T cells; and (4) a step for differentiating the iPS cell clones, the hematopoietic stem cells or the immature T cells, which are obtained in step 3 and into which the cDNAs have been introduced, into mature T cells and proliferating the mature T cells.

Inventors:
KANEKO SHIN (JP)
GONOTSUBO RYOSUKE (JP)
OSAWA MITSUJIRO (JP)
HITOSHI YASUMICHI (JP)
Application Number:
PCT/JP2021/034333
Publication Date:
March 24, 2022
Filing Date:
September 17, 2021
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
THYAS CO LTD (JP)
International Classes:
C12N5/10; A61K35/17; A61P35/00; C12N5/0783; C12N15/12; C12N15/85; C12N15/86
Domestic Patent References:
WO2016010154A12016-01-21
WO2011096482A12011-08-11
WO2020027094A12020-02-06
WO2020022512A12020-01-30
WO2018143454A12018-08-09
WO2021106832A12021-06-03
WO2011096482A12011-08-11
WO2013176197A12013-11-28
Foreign References:
JP2018512380A2018-05-17
Other References:
NISHIMURA T ET AL.: "Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation", CELL STEM CELL, vol. 12, 2013, pages 114 - 126, XP055567898, DOI: 10.1016/j.stem.2012.11.002
MINAGAWA A ET AL.: "Enhancing T cell receptor stability in rejuvenated iPSC-derived T cells improves their use in cancer immunotherapy", CELL STEM CELL, vol. 23, 2018, pages 850 - 858
R. K. LINDEMANN ET AL., MOL. CANCER, vol. 2, 2003, pages 20
TAKAYAMA N. ET AL., J EXP MED., 2010, pages 2817 - 2830
NIWA A ET AL., J CELL PHYSIOL., vol. 221, no. 2, November 2009 (2009-11-01), pages 367 - 77
NIWA A ET AL., PLOS ONE, vol. 6, no. 7, 2011, pages e22261
Attorney, Agent or Firm:
SHA PATENT AND TRADEMARK FIRM (JP)
Download PDF: