Title:
METHOD FOR PRODUCING SIRAITIA GROSVENORII EXTRACT USING IMMOBILIZED ENZYME TECHNOLOGY
Document Type and Number:
WIPO Patent Application WO/2021/169133
Kind Code:
A1
Abstract:
The present invention provides a method for producing a Siraitia grosvenorii extract using immobilized enzyme technology, comprising the following steps: hot water extraction, ceramic membrane filtration, desalination, decoloration, immobilized enzyme enzymolysis, and concentration. If necessary, a part of the enzymatic hydrolysate obtained in step (4) can be filtered by using a nanofiltration membrane, a Siraitia grosvenorii concentrated juice without mogrosides is obtained from the permeate of the nanofiltration membrane, and dry powder of a Siraitia grosvenorii extract is obtained from components retained by the nanofiltration membrane. The method of the present invention can simultaneously produce multiple kinds of liquid and solid Siraitia grosvenorii extract products, thereby satisfying various requirements of the market, and the yield of mogroside V is high. The content of mogroside V in the Siraitia grosvenorii concentrated juice reaches 3 to 8 wt%, and the mass content of mogroside V of the dry powder of the Siraitia grosvenorii extract is 40 to 60 wt%.
Inventors:
LI WEI (CN)
SONG GULIANG (CN)
HUANG HUAXUE (CN)
ZHAO GUANYU (CN)
LIU YONGSHENG (CN)
HUANG JUN (CN)
HE JINJUN (CN)
SONG GULIANG (CN)
HUANG HUAXUE (CN)
ZHAO GUANYU (CN)
LIU YONGSHENG (CN)
HUANG JUN (CN)
HE JINJUN (CN)
Application Number:
PCT/CN2020/100023
Publication Date:
September 02, 2021
Filing Date:
July 03, 2020
Export Citation:
Assignee:
HUNAN HUACHENG BIOTECH INC (CN)
International Classes:
A23L2/39; C07J17/00; A23L2/74; A23L2/84; A23L33/10
Foreign References:
| CN111296708A | 2020-06-19 | |||
| CN107397103A | 2017-11-28 | |||
| CN101167539A | 2008-04-30 | |||
| CN107156563A | 2017-09-15 | |||
| CN106008645A | 2016-10-12 | |||
| JP2016158514A | 2016-09-05 |
Other References:
WANG XIAOHONG, TIAN ZI-QING, HAN RUI: "Enzymatic Hydrolysis Conditions and Application of Mash Enzyme SEB MASH", FARM PRODUCTS PROCESSING, SHANXI ACADEMY OF AGRICULTURAL MECHANIZATION; SHANXI AGRICULTURAL PRODUCTS PROCESSING EQUIPMENT TECHNOLOGY MANAGEMENT STATION; SHANXI AGRICULTURAL INDUSTRIALIZATION ASSOCIATION, CN, no. 6, 15 June 2012 (2012-06-15), CN, pages 30 - 37, XP055840513, ISSN: 1671-9646, DOI: 10.3969/jissn.1671-9646(X).2012.06.008
YU ZHONG: "Novel Mash Enzyme", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, GAI KAN BIANJIBU , BEIJING, CN, vol. 21, no. 6, 15 December 2000 (2000-12-15), CN, pages 56 - 57, XP055840517, ISSN: 1002-0306, DOI: 10.13386/j.issn1002-0306.2000.06.149
JIANG MINGLIAN: "Study on Extraction Technology of Extract of Momordica Grosvenori Swingle", CHINA PHARMACEUTICALS, CHONGQING: ZHONGGUO YAOYE ZAZHISHE, CN, vol. 17, no. 19, 5 October 2008 (2008-10-05), CN, pages 40 - 41, XP055840520, ISSN: 1006-4931
YU ZHONG: "Novel Mash Enzyme", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, GAI KAN BIANJIBU , BEIJING, CN, vol. 21, no. 6, 15 December 2000 (2000-12-15), CN, pages 56 - 57, XP055840517, ISSN: 1002-0306, DOI: 10.13386/j.issn1002-0306.2000.06.149
JIANG MINGLIAN: "Study on Extraction Technology of Extract of Momordica Grosvenori Swingle", CHINA PHARMACEUTICALS, CHONGQING: ZHONGGUO YAOYE ZAZHISHE, CN, vol. 17, no. 19, 5 October 2008 (2008-10-05), CN, pages 40 - 41, XP055840520, ISSN: 1006-4931
Attorney, Agent or Firm:
BEIJING ZHONGZHI XINGYUAN IP LAW FIRM (CN)
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