Title:
METHOD FOR PRODUCING STEROL
Document Type and Number:
WIPO Patent Application WO/2019/078359
Kind Code:
A1
Abstract:
Provided is a method for producing a sterol. A sterol is produced by culturing a microorganism of the class Labyrinthulomycetes, which is capable of producing the sterol and has been modified so as to lower the activity of 24-dehydrocholesterol reductase (DHCR24), 7-dehydrocholesterol reductase (DHCR7) or sterol 24-C-methyltransferase (SMT1), and then collecting the sterol from the culture.
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Inventors:
SUZUKI SHIGEO (JP)
KISELEVA EVGENIYA MIKHAILOVNA (JP)
KISELEVA EVGENIYA MIKHAILOVNA (JP)
Application Number:
PCT/JP2018/039009
Publication Date:
April 25, 2019
Filing Date:
October 19, 2018
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N15/53; A23L33/11; C07J9/00; C12N1/13; C12N15/54; C12P33/00
Domestic Patent References:
| WO2015108058A1 | 2015-07-23 | |||
| WO2017150723A1 | 2017-09-08 | |||
| WO2017065293A1 | 2017-04-20 | |||
| WO2016056610A1 | 2016-04-14 | |||
| WO2016056610A1 | 2016-04-14 | |||
| WO2002083869A2 | 2002-10-24 | |||
| WO2017065293A1 | 2017-04-20 |
Foreign References:
| JP2017153437A | 2017-09-07 | |||
| JP2018064551A | 2018-04-26 | |||
| JP2006304686A | 2006-11-09 |
Other References:
WEETE JD ET AL.: "Lipids and ultrastructure of Thraustochytrium sp. ATCC 26185.", LIPIDS, vol. 32, no. 8, August 1997 (1997-08-01), pages 839 - 45, XP009077836, DOI: 10.1007/s11745-997-0107-z
MYERSMILLER, CABIOS, vol. 4, 1988, pages 11 - 17
SMITH ET AL., ADV. APPL. MATH., vol. 2, 1981, pages 482
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
PEARSONLIPMAN, PROC. NATL. ACAD. SCI., vol. 85, 1988, pages 2444 - 2448
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
HIGGINS ET AL., GENE, vol. 73, 1988, pages 237 - 244
HIGGINS ET AL., CABIOS, vol. 5, 1989, pages 151 - 153
CORPET ET AL., NUCLEIC ACIDS RES., vol. 16, 1988, pages 10881 - 90
HUANG ET AL., CABIOS, vol. 8, 1992, pages 155 - 65
PEARSON ET AL., METH. MOL. BIOL., vol. 24, 1994, pages 307 - 331
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389
MORIKAWA T ET AL.: "Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato", PLANT CELL, vol. 18, no. 4, April 2006 (2006-04-01), pages 1008 - 22
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, 1998, pages 5511 - 5515
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Genetic Engineering", vol. 8, 1987, KYORITSU SHUPPAN CO., LTD, article "Fundamental Microbiology"
GENE, vol. 60, no. 1, 1987, pages 115 - 127
"PCR Technology", 1989, STOCKTON PRESS
KRAMER, W.FRITS, H.J., METH. IN ENZYMOL., vol. 154, 1987, pages 367
NAKAMURA, Y. ET AL.: "Codon Usage Database", NUCL. ACIDS RES., vol. 28, 2000, pages 292, Retrieved from the Internet
GRIMA, E. M. ET AL., BIOTECHNOL. ADVANCES, vol. 20, 2003, pages 491 - 515
MAYUMI UEDA ET AL.: "Seasonal dynamics of culturable thraustochytrids (Labyrinthulomycetes, Stramenopile) in estuarine and coastal waters", AQUATIC MICROBIAL ECOLOGY, vol. 74, 2015, pages 187 - 204
KEISHI SAKAGUCHI ET AL.: "Versatile Transformation system that is Applicable to both multiple transgene expression and gene targeting for thraustochytrids", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 9, 2012, pages 3193 - 3202, XP055148219, DOI: 10.1128/AEM.07129-11
KEISHI SAKAGUCHI ET AL.: "Versatile Transformation system that is Applicable to both multiple transgene expression and gene targeting for thraustochytrids.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 9, 2012, pages 3193 - 3202, XP055148219, DOI: 10.1128/AEM.07129-11
See also references of EP 3699282A4
MYERSMILLER, CABIOS, vol. 4, 1988, pages 11 - 17
SMITH ET AL., ADV. APPL. MATH., vol. 2, 1981, pages 482
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
PEARSONLIPMAN, PROC. NATL. ACAD. SCI., vol. 85, 1988, pages 2444 - 2448
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
HIGGINS ET AL., GENE, vol. 73, 1988, pages 237 - 244
HIGGINS ET AL., CABIOS, vol. 5, 1989, pages 151 - 153
CORPET ET AL., NUCLEIC ACIDS RES., vol. 16, 1988, pages 10881 - 90
HUANG ET AL., CABIOS, vol. 8, 1992, pages 155 - 65
PEARSON ET AL., METH. MOL. BIOL., vol. 24, 1994, pages 307 - 331
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389
MORIKAWA T ET AL.: "Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato", PLANT CELL, vol. 18, no. 4, April 2006 (2006-04-01), pages 1008 - 22
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, 1998, pages 5511 - 5515
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Genetic Engineering", vol. 8, 1987, KYORITSU SHUPPAN CO., LTD, article "Fundamental Microbiology"
GENE, vol. 60, no. 1, 1987, pages 115 - 127
"PCR Technology", 1989, STOCKTON PRESS
KRAMER, W.FRITS, H.J., METH. IN ENZYMOL., vol. 154, 1987, pages 367
NAKAMURA, Y. ET AL.: "Codon Usage Database", NUCL. ACIDS RES., vol. 28, 2000, pages 292, Retrieved from the Internet
GRIMA, E. M. ET AL., BIOTECHNOL. ADVANCES, vol. 20, 2003, pages 491 - 515
MAYUMI UEDA ET AL.: "Seasonal dynamics of culturable thraustochytrids (Labyrinthulomycetes, Stramenopile) in estuarine and coastal waters", AQUATIC MICROBIAL ECOLOGY, vol. 74, 2015, pages 187 - 204
KEISHI SAKAGUCHI ET AL.: "Versatile Transformation system that is Applicable to both multiple transgene expression and gene targeting for thraustochytrids", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 9, 2012, pages 3193 - 3202, XP055148219, DOI: 10.1128/AEM.07129-11
KEISHI SAKAGUCHI ET AL.: "Versatile Transformation system that is Applicable to both multiple transgene expression and gene targeting for thraustochytrids.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 9, 2012, pages 3193 - 3202, XP055148219, DOI: 10.1128/AEM.07129-11
See also references of EP 3699282A4
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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