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Title:
METHOD FOR PURIFYING AND CONCENTRATING DNA IN FORENSIC SAMPLES USING SELECTIVE FILTRATION COLUMN
Document Type and Number:
WIPO Patent Application WO/2020/211637
Kind Code:
A1
Abstract:
A method for purifying and concentrating DNA in forensic samples using a selective filtration column, specifically as follows: adding a cell fluid having undergone digestion and pyrolysis into an inner pipe of a selective filtration column, and at the same time, centrifuging the selective filtration column in a centrifuge; adding 5-100 microliters of water into the selective filtration column, oscillating for 30 seconds, and then transferring the aqueous solution in the selective filtration column to a clean centrifuge tube, and at such time, the solution in the centrifuge tube is a purified and concentrated solution. After using a lysis buffer to perform pyrolysis and digestion on residual human cells in a sample, DNA is then completely free in a solution. Solid particles in the sample are centrifuged away by means of a centrifuge tube, and then by means of an ultrafiltration membrane at a bottom portion of a selective filtration column, said ultrafiltration membrane being permeable to small molecule substances and impermeable to the macromolecule substance that is DNA under a condition of high-speed centrifuging, purification of the macromolecule substance DNA is made possible.

Inventors:
ZHANG DAN (CN)
XIONG HUAI (CN)
LIANG LONGJIE (CN)
Application Number:
PCT/CN2020/082364
Publication Date:
October 22, 2020
Filing Date:
March 31, 2020
Export Citation:
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Assignee:
ANHUI SENPENG BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/10
Foreign References:
CN110129313A2019-08-16
CN110079520A2019-08-02
CN201586570U2010-09-22
EP0517515A21992-12-09
CN2394917Y2000-09-06
CN203513676U2014-04-02
Other References:
YANG DIAN: "Comparison of extraction and purification methods of contact DNA samples and forensic applications", MASTER THESIS, 6 April 2011 (2011-04-06), pages 1 - 76, XP009522714
ZHANG SHAOQING, LIAO YANNI, JIN YANG, GU JIANLI: "Application of Microcon-100 Ultrafiltration Tube in DNA Examination of Exfoliated Epithelial Cells", JOURNAL OF JIANGXI POLICE COLLEGE, vol. 4 (159), 31 July 2012 (2012-07-31), pages 116 - 117, XP009522716, ISSN: 2095-2031
MA QING; TONG MENGJIE; XIA DONGJING: "The application of Amicon Ultra-0.5 ultrafiltration centrifuge tube in DNA extraction from exfoliated cells", JOURNAL OF FORENSIC MEDICINE, vol. 34, no. 2, 30 April 2018 (2018-04-30), pages 189 - 190, XP009522717, DOI: 10.3969/j.issn.1004-5619.2018.02.018
MARTIN FEUERBACH , VOLKER MÜLLER , WOLFGANG SCHWERD : "Ultrafiltration, an Efficient Method for Purification of DNA From Stains", ARCHIV FÜR KRIMINOLOGIE, vol. 187, no. 5-6, 30 June 1991 (1991-06-30), DE, pages 173 - 178, XP009522718
Attorney, Agent or Firm:
NANJING ZHISHI LAW FIRM OF INTELLECTUAL PROPERTY (CN)
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