Title:
A METHOD FOR RECOVERING AN INTRACELLULAR POLYHYDROXYALKANOATE (PHA)
Document Type and Number:
WIPO Patent Application WO/2011/108916
Kind Code:
A3
Abstract:
The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from step (a) with distilled water, (c) freeze- drying the pellet obtained in step (b), (d) adding distilled water to the freeze-dried sample, (e) separating non-PHA cellular material (NPCM) from PHA granules, (f) washing the pellet containing PHA from step (e) by distilled water and centrifuging for producing final pellet, and (g) freeze-drying the pellet produced from step (f).
Inventors:
HASSAN MOHD ALI (MY)
MOHAMMADI MITRA (MY)
AB RAZAK NOR ASMA (MY)
CHONG MEI LING (MY)
ABD AZIZ SURAINI (MY)
ABDULLAH AMIRUL AL-ASHRAF (MY)
CHE MAN HASFALINA (MY)
ANIS SITI NOR SYAIRAH (MY)
PHANG LAI YEE (MY)
SHIRAI YOSHIHITO (MY)
WAN YUNUS WAN MD ZIN (MY)
MOHAMMADI MITRA (MY)
AB RAZAK NOR ASMA (MY)
CHONG MEI LING (MY)
ABD AZIZ SURAINI (MY)
ABDULLAH AMIRUL AL-ASHRAF (MY)
CHE MAN HASFALINA (MY)
ANIS SITI NOR SYAIRAH (MY)
PHANG LAI YEE (MY)
SHIRAI YOSHIHITO (MY)
WAN YUNUS WAN MD ZIN (MY)
Application Number:
PCT/MY2010/000333
Publication Date:
November 24, 2011
Filing Date:
December 30, 2010
Export Citation:
Assignee:
UNIV PUTRA MALAYSIA (MY)
HASSAN MOHD ALI (MY)
MOHAMMADI MITRA (MY)
AB RAZAK NOR ASMA (MY)
CHONG MEI LING (MY)
ABD AZIZ SURAINI (MY)
ABDULLAH AMIRUL AL-ASHRAF (MY)
CHE MAN HASFALINA (MY)
ANIS SITI NOR SYAIRAH (MY)
PHANG LAI YEE (MY)
SHIRAI YOSHIHITO (MY)
WAN YUNUS WAN MD ZIN (MY)
HASSAN MOHD ALI (MY)
MOHAMMADI MITRA (MY)
AB RAZAK NOR ASMA (MY)
CHONG MEI LING (MY)
ABD AZIZ SURAINI (MY)
ABDULLAH AMIRUL AL-ASHRAF (MY)
CHE MAN HASFALINA (MY)
ANIS SITI NOR SYAIRAH (MY)
PHANG LAI YEE (MY)
SHIRAI YOSHIHITO (MY)
WAN YUNUS WAN MD ZIN (MY)
International Classes:
C12P7/62; C12N1/00; C12S3/18
Domestic Patent References:
| WO2010071399A1 | 2010-06-24 |
Foreign References:
| US7070966B2 | 2006-07-04 | |||
| US20080118963A1 | 2008-05-22 | |||
| US7393668B2 | 2008-07-01 | |||
| US20070072276A1 | 2007-03-29 | |||
| US7435567B2 | 2008-10-14 | |||
| US20020031812A1 | 2002-03-14 |
Attorney, Agent or Firm:
DAMODHARAN, Ramakrishna (Suite 8-7-2 Menara Mutiara BangsarJalan Liku Off Jalan Rion, Bangsar Kuala Lumpur, MY)
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