Title:
METHOD FOR REMOVING IMPURITIES BY USING CALCIUM PHOSPHATE PRECIPITATION
Document Type and Number:
WIPO Patent Application WO/2014/185716
Kind Code:
A1
Abstract:
The present invention relates to a method for removing impurities in a sample containing proteins, comprising a step of causing calcium phosphate to precipitate by adding a calcium ion solution and a phosphate ion solution to the sample containing proteins. It is possible to effectively remove impurities such as protein aggregates within a short time and by a simple method if purifying proteins by using the present invention instead of chromatography, which takes a long time and has a complex equipment and method in a purification process after protein A chromatography.
Inventors:
HONG SEUNG SUH (KR)
CHANG SHIN JAE (KR)
KWON KI SUNG (KR)
KWON BYOUNG OH (KR)
JEON KYU JONG (KR)
BAE JIN SOO (KR)
HONG SU HYEON (KR)
CHANG SHIN JAE (KR)
KWON KI SUNG (KR)
KWON BYOUNG OH (KR)
JEON KYU JONG (KR)
BAE JIN SOO (KR)
HONG SU HYEON (KR)
Application Number:
PCT/KR2014/004341
Publication Date:
November 20, 2014
Filing Date:
May 15, 2014
Export Citation:
Assignee:
CELLTRION INC (KR)
International Classes:
C07K1/16; C07K1/34; C07K16/00; C07K19/00
Foreign References:
| US8263750B2 | 2012-09-11 | |||
| KR20050119380A | 2005-12-21 |
Other References:
ZHANG ET AL.: "Highly efficient phosphopeptide enrichment by calcium phosphate precipitation combined with subsequent IMAC enrichment", MOLECULAR & CELLULAR PROTEOMICS, vol. 6, no. 11, 2007, pages 2032 - 2042
PEPYS ET AL.: "Isolation of C-reactive protein by affinity chromatography", CLINICAL AND EXPERIMENTAL IMMUNOLOGY, vol. 30, no. 1, 1997, pages 32 - 37
SCHLESINGER ET AL.: "Complete covalent structure of statherin, a tyrosine-rich acidic peptide which inhibits calcium phosphate precipitation from human parotid saliva", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 252, no. 5, 1997, pages 1689 - 1695
PEPYS ET AL.: "Isolation of C-reactive protein by affinity chromatography", CLINICAL AND EXPERIMENTAL IMMUNOLOGY, vol. 30, no. 1, 1997, pages 32 - 37
SCHLESINGER ET AL.: "Complete covalent structure of statherin, a tyrosine-rich acidic peptide which inhibits calcium phosphate precipitation from human parotid saliva", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 252, no. 5, 1997, pages 1689 - 1695
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