Title:
METHOD FOR SELECTING SKELETAL MUSCLE PROGENITOR CELL
Document Type and Number:
WIPO Patent Application WO/2016/114354
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for selecting a skeletal muscle progenitor cell from a cell group including skeletal muscle progenitor cells. The problem is solved by providing a step for introducing miRNA-responsive mRNA into the cell group. The miRNA-responsive mRNA contains: (i) a nucleic acid containing a sequence specifically recognized by miRNA that is specifically expressed in skeletal muscle progenitor cells; and (ii) a nucleic acid containing a sequence that codes for a marker protein.
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Inventors:
SAITO HIROHIDE (JP)
TAKAHASHI SEIYA (JP)
SAKURAI HIDETOSHI (JP)
SATO TAKAHIKO (JP)
TAKAYAMA SATORU (JP)
TAKAHASHI SEIYA (JP)
SAKURAI HIDETOSHI (JP)
SATO TAKAHIKO (JP)
TAKAYAMA SATORU (JP)
Application Number:
PCT/JP2016/050997
Publication Date:
July 21, 2016
Filing Date:
January 14, 2016
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/10; C12N15/00; C12N15/09
Domestic Patent References:
| WO2010008100A1 | 2010-01-21 | |||
| WO2013073246A1 | 2013-05-23 | |||
| WO2011154553A2 | 2011-12-15 | |||
| WO2015141827A1 | 2015-09-24 |
Foreign References:
| US20100323356A1 | 2010-12-23 |
Other References:
SHELTON, MICHAEL ET AL.: "Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells", STEM CELL REPORTS, vol. 3, no. 3, 2014, pages 516 - 529, XP055281322, DOI: doi:10.1016/j.stemcr.2014.07.001
TOWNLEY-TILSON ET AL.: "MicroRNAs 1, 133, and 206: Critical factors of skeletal and cardiac muscle development, function, and disease", THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, vol. 42, 2010, pages 1252 - 1255, XP002601173, DOI: doi:10.1016/J.BIOCEL.2009.03.002
MIKI, KENJI ET AL.: "Efficient Detection and Purification of Cell Populations UsingSynthetic MicroRNA Switches", CELL STEM CELL, vol. 16, pages 699 - 711, XP055225629, DOI: doi:10.1016/j.stem.2015.04.005
See also references of EP 3246398A4
TOWNLEY-TILSON ET AL.: "MicroRNAs 1, 133, and 206: Critical factors of skeletal and cardiac muscle development, function, and disease", THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, vol. 42, 2010, pages 1252 - 1255, XP002601173, DOI: doi:10.1016/J.BIOCEL.2009.03.002
MIKI, KENJI ET AL.: "Efficient Detection and Purification of Cell Populations UsingSynthetic MicroRNA Switches", CELL STEM CELL, vol. 16, pages 699 - 711, XP055225629, DOI: doi:10.1016/j.stem.2015.04.005
See also references of EP 3246398A4
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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