METHOD FOR SEPARATING GRAM-NEGATIVE BACTERIUM-DERIVED INTACT PROTEIN

Title:

METHOD FOR SEPARATING GRAM-NEGATIVE BACTERIUM-DERIVED INTACT PROTEIN

Document Type and Number:

WIPO Patent Application WO/2022/164224

Kind Code:

A2

Abstract:

The present invention relates to a method for eluting a prokaryotic protein. In the present invention, a protein can be obtained in the intact form with no damages thereto by destructing cell walls of bacteria through a simple process of adding an organic solvent including a lower alcohol or a nitrile derivative to a sample including pathogenic bacteria, etc. or applying an osmotic stimulus to the sample. The present invention can be advantageously used for rapidly and accurately identifying periplasmic proteins of, among others, Gram-negative bacteria without additional purification processes.

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Inventors:

CHUN JONG KEE (KR)
BAEK JE HYUN (KR)
YANG WON SUK (KR)
LEE SAEYOUNG (KR)
CHEON DONG HUEY (KR)
JANG HEEJUNG (KR)
HWANG SEOHYUN (KR)

Application Number:

PCT/KR2022/001480

Publication Date:

August 04, 2022

Filing Date:

January 27, 2022

Export Citation:

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Assignee:

SEEGENE MEDICAL FOUND (KR)

International Classes:

C07K1/12

Attorney, Agent or Firm:

PADO IP LAW PLLC (KR)

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