METHOD OF SEPARATING AND PURIFYING ICARIIN FROM EPIMEDIUM EXTRACT

WIPO Patent Application WO/2019/241947

A1

Disclosed is a method of separating and purifying icariin from Epimedium extract, relating to the technical field of separating and purifying a single component from a plant extract, and is intended to address the issues of complicated processing, high preparation costs, and environmental pollution in existing methods. The method comprises: 1) blending an Epimedium extract with an aqueous acetone solution having a volume fraction of 88%-96%; 2) filtering and collecting a filter cake, and then dissolving the filter cake in an aqueous ethanol solution having a volume fraction of 50%-75%; 3) after the dissolution is completed, filtering and collecting the filtrate, and concentrating the filtrate to remove the ethanol, thereby obtaining a concentrated liquid; 4) at a first temperature, adding acetone to the concentrated liquid, mixing uniformly, and lowering the temperature to a second temperature after sufficient dissolution to perform crystallization; 5) after the crystallization is completed, filtering and collecting a filter cake, and letting the filter cake dry to obtain crude icariin. The method can provide a single component having a icariin content of more than 98% that satisfies the high purity requirement of injection.

FU RONGZHAO (CN)
LIU LIHUI (CN)
GUO XINGLIN (CN)
CHEN XIAOCHUN (CN)

PCT/CN2018/092098

December 26, 2019

June 21, 2018

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BONTAC BIO ENG SHENZHEN CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)

C07H17/07; C07H1/08

CN106589020A	2017-04-26
CN101812100A	2010-08-25
CN102070688A	2011-05-25
CN102617670A	2012-08-01
CN103396463A	2013-11-20

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