Title:
METHOD FOR SMALL-RNA BIOMARKER IDENTIFICATION AND FUNCTIONAL EVALUATION OF CIRCULATING EXTRACELLULAR VESICLES COMPRISING EXOSOMES
Document Type and Number:
WIPO Patent Application WO/2022/140662
Kind Code:
A3
Abstract:
A method of purifying Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER), designed for high-throughput analysis of low- abundance small-RNA cargos by next-generation sequencing, and use of this method for preparing purified populations of biological particles or cells from a biological sample for in vitro evaluation of a patient's risk of developing and for treating a severe viral infection; for enhancing therapeutic effectiveness of convalescent plasma therapy in a patient at risk for a severe coronavirus infection, and for treating a patient with a severe coronavirus infection, are described.
Inventors:
MITCHELL MEGAN (US)
LOUDIG OLIVIER (US)
KAMALI-MOGHADDAM MASOOD (SE)
LOUDIG OLIVIER (US)
KAMALI-MOGHADDAM MASOOD (SE)
Application Number:
PCT/US2021/065060
Publication Date:
August 04, 2022
Filing Date:
December 23, 2021
Export Citation:
Assignee:
HACKENSACK MERIDIAN HEALTH CENTER FOR DISCOVERY AND INNOVATION (US)
International Classes:
G01N33/50; C07K1/22; C07K16/00; G01N33/53; H01J49/26
Foreign References:
| US20130123121A1 | 2013-05-16 | |||
| US20190219578A1 | 2019-07-18 | |||
| US20180002695A1 | 2018-01-04 |
Other References:
NIU FUZHOU, CHEN XIFU, NIU XUEMEI, CAI YIFAN, ZHANG QINGKUI, CHEN TAO, YANG HAO: "Integrated Immunomagnetic Bead-Based Microfluidic Chip for Exosomes Isolation", MICROMACHINES, vol. 11, no. 5, 15 May 2020 (2020-05-15), XP055958444, DOI: 10.3390/mi11050503
YAN ET AL.: "A universal approach to prepare reagents for DNA-assisted protein analysis", PLOS ONE, vol. 9, no. 9, 18 September 2014 (2014-09-18), pages 1 - 8, XP055820699, DOI: 10.1371/journal.pone.0108061
GONG ET AL.: "Simple Method To Prepare Oligonucleotide-Conjugated Antibodies and Its Application in Multiplex Protein Detection in Single Cells", BIOCONJUG CHEM, vol. 27, no. 1, 20 January 2016 (2016-01-20), pages 217 - 225, XP055408616, DOI: 10.1021/acs.bioconjchem.5b00613
EL-SHENNAWY LAMIAA, HOFFMANN ANDREW D., DASHZEVEG NURMAA K., MEHL PAUL J., YU ZIHAO, TOKARS VALERIE L., NICOLAESCU VLAD, OSTIGUIN : "Circulating ACE2-expressing Exosomes Block SARS-CoV-2 Infection as an Innate Antiviral Mechanism", BIORXIV, 4 December 2020 (2020-12-04), XP055958450, [retrieved on 20220907], DOI: 10.1101/2020.12.03.407031
YAN ET AL.: "A universal approach to prepare reagents for DNA-assisted protein analysis", PLOS ONE, vol. 9, no. 9, 18 September 2014 (2014-09-18), pages 1 - 8, XP055820699, DOI: 10.1371/journal.pone.0108061
GONG ET AL.: "Simple Method To Prepare Oligonucleotide-Conjugated Antibodies and Its Application in Multiplex Protein Detection in Single Cells", BIOCONJUG CHEM, vol. 27, no. 1, 20 January 2016 (2016-01-20), pages 217 - 225, XP055408616, DOI: 10.1021/acs.bioconjchem.5b00613
EL-SHENNAWY LAMIAA, HOFFMANN ANDREW D., DASHZEVEG NURMAA K., MEHL PAUL J., YU ZIHAO, TOKARS VALERIE L., NICOLAESCU VLAD, OSTIGUIN : "Circulating ACE2-expressing Exosomes Block SARS-CoV-2 Infection as an Innate Antiviral Mechanism", BIORXIV, 4 December 2020 (2020-12-04), XP055958450, [retrieved on 20220907], DOI: 10.1101/2020.12.03.407031
Attorney, Agent or Firm:
LUBIT, Beverly W. et al. (US)
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