Title:
METHOD AND SYSTEM FOR IDENTIFICATION OF TARGET SITES IN PROTEIN CODING REGIONS FOR COMBATING PATHOGENS
Document Type and Number:
WIPO Patent Application WO/2020/245783
Kind Code:
A3
Abstract:
A method and system for identification of target sites in protein coding regions for combating pathogens has been provided. The method relates to identifying a set of nucleotide repeat sequences that occur within the complete coding region of a specific protein that is involved in the pathogenicity of the infectious bacteria and which occurs in multiple copies on the pathogen genome and utilizing various laboratory acceptable methods to debilitate the identified target sequence on the pathogen genome, as well as use of enzymatic machinery to target and cleave its flanking genes on the genome. The set of nucleotide repeat sequences forming a part of or the complete coding region of a specific protein on the pathogen genome may be flanked by genes on either side that can be targeted as well. The present disclosure further includes administration of a cocktail comprising antimicrobial drugs, biofilm inhibitors and the novel construct.
Inventors:
MANDE SHARMILA SHEKHAR (IN)
ANAND SWADHA (IN)
SAMPATH PREETHI ALAGARAI (IN)
ANAND SWADHA (IN)
SAMPATH PREETHI ALAGARAI (IN)
Application Number:
PCT/IB2020/055308
Publication Date:
May 14, 2021
Filing Date:
June 05, 2020
Export Citation:
Assignee:
TATA CONSULTANCY SERVICES LTD (IN)
International Classes:
G16B40/00; A61K38/00; A61K39/00; A61P31/00; A61P31/04; G16B25/00
Domestic Patent References:
| WO2015184259A1 | 2015-12-03 |
Foreign References:
| US20190021343A1 | 2019-01-24 | |||
| US20120004111A1 | 2012-01-05 |
Other References:
BHATT ET AL.: "FLIM-MAP: Gene Context Based Identification of Functional Modules in Bacterial Metabolic Pathways", FRONTIERS IN MICROBIOLOGY, vol. 9, no. 2183, 18 September 2018 (2018-09-18), pages 1 - 11, XP055822403
GOMAA ET AL.: "Programmable Removal of Bacterial Strains by Use of Genome-Targeling CRISPR-Cas Systems", MBIO, vol. 5, no. 1, 28 January 2014 (2014-01-28), pages 1 - 9, XP055227960
PENG ET AL.: "EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens", MICROBIAL GENOMICS, vol. 1, no. 4, 30 October 2015 (2015-10-30), pages 1 - 7, XP055681566, DOI: 10.1099/mgen.0.000033
GOMAA ET AL.: "Programmable Removal of Bacterial Strains by Use of Genome-Targeling CRISPR-Cas Systems", MBIO, vol. 5, no. 1, 28 January 2014 (2014-01-28), pages 1 - 9, XP055227960
PENG ET AL.: "EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens", MICROBIAL GENOMICS, vol. 1, no. 4, 30 October 2015 (2015-10-30), pages 1 - 7, XP055681566, DOI: 10.1099/mgen.0.000033
Attorney, Agent or Firm:
KHAITAN & CO (IN)
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