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Title:
METHODS OF CULTURING ANIMAL CELLS AND POLYPEPTIDE PRODUCTION IN ANIMAL CELLS
Document Type and Number:
WIPO Patent Application WO2002101019
Kind Code:
A3
Abstract:
A method of improving expression of polypeptides in mammalian cell culture systems is provided. In particular, the invention is directed to methods of culturing mammalian cells under conditions such as, for example, high levels of glucose, where environmental and nutritional conditions are controlled and adjusted so as to improve process robustness and to promote superior expression of polypeptides having desired qualities, e.g., biological activity. The invention also relates to polypeptides, especially antibodies, produced by the methods, and to methods of using such antibodies, and to pharmaceutical compositions containing the polypeptides. The invention also relates to methods of culturing animal cells in a fed batch cell culture to maximize production capacity, viability of the cell culture, and overall enhancement of yield from such cultures.

Inventors:
DELACRUZ NESLIHAN (US)
ETCHEVERRY TINA (US)
OSTLAND MICHAEL (US)
Application Number:
PCT/US2002/018687
Publication Date:
April 10, 2003
Filing Date:
June 11, 2002
Export Citation:
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Assignee:
GENENTECH INC (US)
DELACRUZ NESLIHAN (US)
ETCHEVERRY TINA (US)
OSTLAND MICHAEL (US)
International Classes:
A61K39/395; C07K16/00; C07K16/18; C12N5/02; C12N5/06; C12N15/09; C12P21/02; C12P21/04; C12P21/08; (IPC1-7): C12N5/02; C12N5/00; C12N15/09; C12P21/06; C12P21/04
Foreign References:
US5672502A1997-09-30
US6156570A2000-12-05
US5721121A1998-02-24
Other References:
See also references of EP 1404813A4
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