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Title:
METHODS OF CULTURING A MAMMALIAN CELL
Document Type and Number:
WIPO Patent Application WO/2016/106192
Kind Code:
A3
Abstract:
Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Inventors:
HWANG CHRISTOPHER (US)
JOHNSON TIMOTHY (US)
WALTHER JASON (US)
CHENG CHENG (US)
WANG JONATHAN (US)
SHAH NEHA (US)
BAE SEUL-A (US)
Application Number:
PCT/US2015/067040
Publication Date:
November 03, 2016
Filing Date:
December 21, 2015
Export Citation:
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Assignee:
GENZYME CORP (US)
International Classes:
C12N5/00
Domestic Patent References:
WO2015188009A12015-12-10
Foreign References:
Other References:
KARIN TÖRDAHL ET AL: "Study of a perfusion process of Chinese Hamster Ovary cells by ATF filtration in bioreactor Ovary cells by ATF filtration in bioreactor", 11 September 2009 (2009-09-11), XP055274145, Retrieved from the Internet [retrieved on 20160520]
MANOHAR KATAKAM ET AL: "Effect of Surfactants on the Physical Stability of Recombinant Human Growth Hormone", JOURNAL OF PHARMACEUTICAL SCIENCES, AMERICAN PHARMACEUTICAL ASSOCIATION, WASHINGTON, US, vol. 84, no. 6, 1 June 1995 (1995-06-01), pages 713 - 716, XP002114576, ISSN: 0022-3549
Attorney, Agent or Firm:
REITER, Tiffany (P.O. Box 1022Minneapolis, Minnesota, US)
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