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Patent Searching and Data


Title:
METHODS FOR IDENTIFICATION AND USES OF ANTI-INFLAMMATORY RECEPTORS FOR EICOSAPENTAENOIC ACID ANALOGS
Document Type and Number:
WIPO Patent Application WO2004078143
Kind Code:
A3
Abstract:
The present invention is directed to methods for the identification and uses of a receptors that interact with anti-inflammatory compounds derived from eicosapentaenoic acid (EPA). The receptors are of the G-protein coupled receptor (GPCR) family, and are useful to screen candidate substances for anti-inflammatory activity, especially substances that are analogs of EPA. Such analogs are termed "resolvins"; and are typically di- and tri-hydroxy EPA analogs. One analog herein denoted Resolvin E1 was identified in humans and prepared by total synthesis. In nanomolar range Resolvin E1 reduces dermal inflammation, peritonitis, dendritic cells (DCs) migration and IL-12 production. Also described herein is a receptor denoted Reso ER1 that interacts with Resolvin E1 to attenuate cytokine induced activation of inflammatory pathways mediated by transcription factor (NF)-kB. Treatment of DCs with small-interfering RNA specific for ResoE1 eliminated the ligand's ability to regulate IL-12. Assays of anti-inflammatory activity based on these discoveries are also described.

Inventors:
SERHAN CHARLES N (US)
ARITA MAKOTO (US)
Application Number:
PCT/US2004/006766
Publication Date:
December 21, 2006
Filing Date:
March 05, 2004
Export Citation:
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Assignee:
BRIGHAM & WOMENS HOSPITAL (US)
SERHAN CHARLES N (US)
ARITA MAKOTO (US)
International Classes:
G01N33/53; C12N15/12; G01N33/50; G01N33/567; A61K
Other References:
SERHAN C.N. ET AL: "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals.", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 196, no. 8, 21 October 2002 (2002-10-21), TOKYO, JAPAN, pages 1025 - 1037, XP002267881
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